IC4R015-RNA-Seq-2010-20086188
Contents
Project Title
- Global Epigenetic and Transcriptional Trends among Two Rice Subspecies and Their Reciprocal Hybrids
The Background of This Project
- The behavior of transcriptomes and epigenomes in hybrids of heterotic parents is of fundamental interest. In this project, the researchers reported highly integrated maps of the epigenome, mRNA, and small RNA transcriptomes of two rice (Oryza sativa) subspecies and their reciprocal hybrids.
Plant Culture & Treatment
- Rice cultivar Nipponbare (Oryza sativa ssp japonica) and 93-11 (O. sativa ssp indica) and their reciprocal F1 hybrids (Nipponbare/93-11 and 93-11/ Nipponbare) were used for all experiments in this study. Seeds were grown in soil under 16-h-light/8-h-dark conditions at 288C in a greenhouse. After 4 weeks, seedling shoots at the four-leaf stage were harvested, frozen in liquid nitrogen, and stored at –808C for DNA and total RNA isolation or processed directly after harvesting for ChIP assay.
Illumina Sequencing
Research Findings
Labs working on this Project
- Peking-Yale Joint Center of Plant Molecular Genetics and Agrobiotechnology, National Laboratory of Protein Engineering and
Plant Genetic Engineering, College of Life Sciences, Peking University, Beijing 100871, China
- National Institute of Biological Sciences, Beijing 102206, China
- Department of Molecular, Cellular, and Developmental Biology, Yale University, New Haven, Connecticut 06520
- Institute of Biophysics, Chinese Academy of Sciences, Beijing 100101, China
- Department of Botany, College of Life Sciences, Hunan Normal University, Changsha 410081, China
Corresponding Author
- xingwang.deng@yale.edu
