IC4R007-lncRNA-2015-26437919

Project Title

• Genome-wide identification of long noncoding RNA genes and their potential association with fecundity and virulence in rice brown planthopper, Nilaparvata lugens

The Background of This Project

• The functional repertoire of long noncoding RNA (lncRNA) has been characterized in several model organisms, demonstrating that lncRNA plays important roles in fundamental biological processes. However, they remain largely unidentified in most species. Understanding the characteristics and functions of lncRNA in insects would be useful for insect resources utilization and sustainable pest control.

Plant Culture & Treatment

• The rice brown planthoppers were collected from rice fields in Nanjing area, Jiangsu Province, China and maintained on rice seedlings at 27 ± 1 °C, under a 16-h light/8-h dark photoperiod and 70–80 % relative humidity. The insects were transferred to fresh seedlings every 5–7 days to ensure sufficient nutrition.

Research Findings

• A computational pipeline was developed to identify lncRNA genes from the N. lugens transcriptome (Fig. 1).This pipeline was applied on 12 different N. lugens transcriptome datasets and yielded 2439 transcript isoforms corresponding to 1882 loci from 12 N. lugens RNA-seq datasets.

'Fig. 1 The computational pipeline for identifying lncRNA genes in N. lugens from RNA-seq data.'

Labs working on this Project

• Department of Entomology, College of Plant protection, Nanjing Agricultural University,Nanjing 210095, China.
• Department of City Construction, Shaoyang University, Shaoyang 422000, China.
• State Key Laboratory for Biocontrol/Institute of Entomology, Sun Yat Sen University,Guangzhou 510275, China.
• Ministry of Agriculture Key Lab of Agricultural Entomology, Institute of Insect Sciences, Zhejiang University, 866 Yuhangtang Road, Hangzhou 310058, China.

Corresponding Author

• lifei18@zju.edu.cn