IC4R005-lncRNA-2016-19769571

Project Title

Novel drought-responsive regulatory coding and non-coding transcripts from Oryza Sativa L.

Drought-stress can cause major economic loss and is a serious issue to address in agriculture. Defining the molecular pathways in how a plant responds to droughtstress may prove valuable in developing new drought-resistant plants. In this study, the researchers identified several novel drought-responsive regulatory coding and noncoding transcripts in rice, Oryza sativa L., using the next generation sequencing (NGS) technique and bioinformatics analyses.

Rice plants, O.sativa L. Nipponbare, were acquired from the Rural Development Administration of Korea (RDA). The rice plants were cultured in Yoshida solution at pH 5.8 and maintained in a temperaturecontrolled culture room at 29 ℃ under 16 h/8 h light/dark conditions. Rice plants at the three-leaf stage were subjected to drought-stress for 1 and 6 h by removal of the culture solution. Untreated plants were used as control. After treatment, entire plants were immediately transferred into liquid nitrogen.

Department of Biomedical Sciences, Sunmoon University, Asan 336-708, Korea
Department of Computer Science and Engineering, Seoul National University, Gwanak-Gu, Seoul 151-744, Republic of Korea
Interdisciplinary Program in Bioinformatics, Seoul National University, Gwanak-Gu, Seoul 151-747, Republic of Korea
Bioinformatics Institute, Seoul National University, GwanakGu, Seoul 151-747, Republic of Korea