IC4R011-GWAS-2016-26381647

Project Title

• New insights into the genetic basis of natural chilling and cold shock tolerance in rice by genome-wide association analysis

The Background of This Project

• As one of the major crops, rice is widely grown in tropical, subtropical and temporal regions, and temperature is one of the major environmental factors limiting its geographic distribution. The optimal temperature for rice growth is 25–30°C (Kim et al. 2014).Previous studies on low-temperature stress in rice mainly concentrated on chilling stress (temperature around 10°C), which was frequently used to distinguish it from freezing stress (temperature around 0°C); however, there is no clear definition of chilling or cold/freezing stress, and the treatment temperatures were often different for the same term in many reports (Cheng et al. 2007;Guo et al. 2006; Ma et al. 2015; Wang et al. 2013; Wang et al.2014; Yang et al. 2012). To date, no report has compared different low-temperature stresses such as natural chilling stress with the acute freezing stress in rice. Recent studies have revealed some mechanisms and signalling networks involved in the cold stress response in rice (Knight & Knight 2012; Ma et al. 2015; Wang et al. 2014; Yang et al. 2012).

Plant Culture & Treatment

• A total of 529 rice accessions including 202 from the China Core Collection and 327 from the World Core Collection were used for the association analysis (Supporting Information Table S1). This panel of rice accessions is essentially the same as the panel of 533 accessions as previously described except three accessions (C126, W196 and W232) with severe heterozygosity and one (W190) with a low mapping rate (10%) omitted.
• After germination for 7 d, the seedlings with uniform growth were transplanted to 10 cm × 10 cm pots each containing nine seedlings. Each accession was planted in three pots as three biological repeats, and the layout of the pots followed a completely randomized block design.
• In this study, we designed two treatments to mimic the natural chilling (temperature gradually declined to a range of 5–12 °C)

and cold shock (constant temperature at 4 °C) stresses, respectively. For the natural chilling stress treatment, the seedlings were grown in a greenhouse for 3 weeks, at which time the plants went into the 4-leaf-stage. The natural chilling treatment was carried out in the greenhouse in winter (Wuhan, China) with the heating and light turned off and the natural low-temperature fluctuating between 5 and 12 °C depending on the outside temperature. The temperature in the greenhouse was recorded every half hour by a weather station (Spectrum Technologies,Inc. WatchDog 2000), and a portion of the record is provided in Supporting Information Fig. S1. For the cold shock treatment, the 4-leaf-stage rice plants were performed in a growth chamber set at a constant 4 °C with 14/10 h of light/darkness.

Labs working on this Project

• National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, Wuhan 430070, China
• National Center of Plant Gene Research (Wuhan), Huazhong Agricultural University, Wuhan 430070, China

Corresponding Author

• Lizhong Xiong:lizhongx@mail.hzau.edu.cn