IC4R009-GWAS-2016-26860200

Project Title

Genome-wide prediction models that incorporate de novo GWAS are a powerful new tool for tropical rice improvement

In all, 369 elite breeding lines (F6–F7) were selected for genotyping from the IRRI irrigated rice breeding program based on the planned inclusion of the lines in the 2011 Multi-Environment Testing Program and presence in the 2011 (Los Baños).

Two phenotype data sets were used in this study, (1) the RYT data set, consisting of field data from 2009–2012, two seasons per year (dry season (DS) and wet season (WS)) collected in a single field at IRRI in Los Baños, Philippines, and (2) the MET data set, consisting of field data from 2011 and 2012, two season per year (dry and wet), at a total of eight sites in SE Asia (Table 1).

For the RYT data set, phenotypes collected included plant height, flowering time, maturity date, number of effective tillers or panicles per plant, lodging score, grain yield and rep number (Supplementary Materials and Methods).

For the MET data set, in addition to the phenotypes collected for the RYT data set, data were collected on field row, field column, phenotypic acceptability score for whole plant, phenotypic acceptability score for panicle and phenotypic acceptability score for grain (Supplementary Materials and Methods). The eight sites at which the MET data were collected to compose IRRI's target population for irrigated rice in SE Asia including IRRI/Los Baños (‘MET field’), Isabela, Nueva Ecija, Agusan del Norte, Bohol, and Midsayap—all in the Philippines, Batalagoda, Sri Lanka, and Hai Dong, Vietnam. Data were highly unbalanced (Table 1).

Table 1 Summary of the two data sets used in this study.

Department of Plant Breeding and Genetics, 240 Emerson Hall, Cornell University, Ithaca, NY, USA;
Department of Plant Breeding, Genetics and Biotechnology, International Rice Research Institute, Los Baños, Philippines
USDA-ARS, North Atlantic Ares, Robert W. Holley Center for Agriculture and Health, Ithaca, NY, USA
Current address: Bangladesh Rice Research Institute, Gazipur 1701, Bangladesh.
Current address: Delta Research and Extension Center, 82 Stoneville Road, PO Box 197, Stoneville, MS 38776, USA.