IC4R007-Phenomics-2015-26104586

Project Title

• Field phenomics for response of a rice diversity panel to ten environments in Senegal and Madagascar. 2. Chilling-induced spikelet sterility

Plant Culture & Treatment

• The panel was a sub-sample of the ORYTAGE species-wide (O.sativa L.) diversity panel of Cirad (http://ricephenonetwork.irri.org/ diversity-panels/orytage-diversity-panels). It was composed of 200 indica accessions, augmented for comparative purposes with 22 accessions representing other genetic groups (3 aus, 3 temperate japonica, 14 tropical japonica, 2 aromatic). The indica population

covered improved and traditional varieties from all tropical regions but had large sub-populations from Madagascar (36) and W-Africa(48, thereof 31 improved lines from AfricaRice bred in Senegal) to capture adaptations to the climatic constraints at the experimental sites. Twenty-one improved varieties and lines were from IRRI(Philippines). A complete list of accessions’ geographic origin and seed sources is presented in Table S1. The 203 accessions of the indica sub-species (200 indica and 3 aus) were genotyped with 825 single nucleotide polymorphism (SNP) markers well distributed in the genome and submitted to a genetic structure analysis using the software Structure v 2. 3. 1 (Pritchard et al., 2000), following a methodology similar to that described in detail in Courtois et al.(2013) for another rice panel. Accessions that had more than 66% of their genome coming from a given sub-group were assigned to this sub-group. The assignment of the accessions to the four sub-groups numbered I1 to I4 that were identified in the panel is indicated in Table S1. The other accessions, which were intermediate between sub-groups, were considered as admixed (Im).

Labs working on this Project

• Cirad, Umr AGAP (Department BIOS) and Upr AIDA (Department ES), F-34398 Montpellier, France
• IRRI, CESD Division, DAPO Box 7777, Metro Manila, Philippines
• Université d’Antananarivo, Département de Biologie et Ecologie Végétales, BP 906, Antananarivo 101, Madagascar
• SRR FOFIFA, BP 230, Antsirabe 110, Madagascar
• Africa Rice Center, Sahel Station, P.B. 96, St. Louis, Senegal

Corresponding Author

• M. Dingkuhn:m.dingkuhn@irri.org