IC4R007-Epigenomic-2014-26432592

Project Title

• Comparison of space flight and heavy ion radiation induced genomic/epigenomic mutations in rice(Oryzasativa)

Plant Materials & Treatment

• Seeds of rice(Oryzasativa,Japonica) were aboard 20th recoverable satellite of China for an 18 day(from September 27 to October 15, 2004) space flight. A total of 200 rice seeds were packed in cotton sack and fixed inside the satellite. The same number of rice seeds were also packed in cotton sack and kept under nor-mal ambient conditions, to be used as ground control. After space flight, the seeds were germinated under standard conditions together with the controls.
• Seeds of rice(Oryzasativa,Japonica) were positioned in the chamber which was fixed to the irradiation equipment at the Heavy Ion Research Facility in Lanzhou (HIRFL). Rice seeds were radiated with 12C6+ ion beam. For the first radiation group (R1), 150 rice seeds were treated and the mean linear energy transfer (LET)within the seeds was calculated to be 62.2 KeV/μm. The dose and dose rate were 200 mGy and 0.2 Gy/min. For the second radiation group (R2), 150 rice seeds were treated and the mean LET within the seeds was calculated to be 27.4 KeV/μm. The dose and dose rate were 2 Gy and 0.1 Gy/min. For each radiation assay, the same number of rice seed placed on the same condition with dose of 0 Gy were used as controls.
• After space flight and ground radiation treatment, rice seeds were germinated under standard conditions and then cultured on trial field for 18 weeks. The controls were cultured at the same conditions. Leaves of the mature plants were obtained and stored at −80℃, until further use. For heredity examination, the selected individuals were tagged and analyzed later. Panicles of the tagged plants were bagged, and seeds were collected from each individual plant and designated as the progenies. The progenies were planted on the same trial field next year.

Labs working on this Project

• CollegeofLifeScience,NortheastForestryUniversity,Harbin,PRChina
• InstituteofExtremeEnvironmentNutritionandProtection,SchoolofFoodScienceandEngineering,HarbinInstituteofTechnology,Harbin,PRChina
• InstituteofEnvironmentalSystemsBiology,DalianMaritimeUniversity,Dalian,PRChina

Corresponding Author

• Y.Sun:yqsun@dlmu.edu.cn