IC4R008-Metabolomics-2014-25057267
Contents
Project Title
- Toward better annotation in plant metabolomics: isolation and structure elucidation of 36 specialized metabolites from Oryza sativa (rice) by using MS/MS and NMR analyses
The Background of This Project
- Metabolomics plays an important role in phytochemical genomics and crop breeding; however, metabolite annotation is a significant bottleneck in metabolomic studies. In particular, in liquid chromatography–mass spectrometry (MS)-based metabolomics, which has become a routine technology for the profiling of plant-specialized metabolites, a substantial number of metabolites detected as MS peaks are still not assigned properly to a single metabolite. Oryza sativa (rice) is one of the most important staple crops in the world. In the present study, the researchers isolated and elucidated the structures of specialized metabolites from rice by using MS/MS and NMR.
Plant Materials & Treatment
- Rice plants (cultivar Habataki) were grown in plastic pots containing granular soil (Bonsoru No.2; Sumitomo Chemical, Tokyo); after approximately 10 weeks of incubation, shoots were collected, lyophilized, and stored at -80 ℃ until use (Matsuda et al. 2012).
Research Findings
- 36 compounds, including five new flavonoids (6–9 and 24), were isolated and assigned from the leaves of rice using MS/MS and NMR methods (Fig. 1). To our knowledge, this is the first time that 18 of the known compounds (4, 5, 12, 13, 17–23, 29–33, 35, and 36) have been isolated from rice leaves.
Labs working on this Project
- RIKEN Center for Sustainable Resource Science, 1-7-22
- School of Pharmacy, Nihon University, 7-7-1 Narashinodai
- School of Pharmacy, Showa University, 1-5-8 Hatanodai
- Graduate School of Pharmaceutical Sciences, Chiba University
Corresponding Author
- K. Saito: kazuki.saito@riken.jp
