IC4R009-Metabolomics-2013-25056584

Project Title

• Comparative metabolic profiling of pigmented rice (Oryza sativa L.) cultivars reveals primary metabolites are correlated with secondary metabolites

Plant Materials & Treatment

• Six cultivars of black rice (Heugjinjubyeo, BR-1; Heugseolbyeo, BR-2; Heugkwangbyeo, BR-3; Josengheugchalbyeo, BR-4; Heugnambyeo, BR-5; Heughyangbyeo, BR-6) and one of white rice (Hwasungbyeo, WR-1) were used. Previously, we reported the

flavonoid and carotenoid contents in these cultivars, which might provide new opportunities for rice breeders and eventually commercial rice growers to promote the production of rice with enhanced nutritional quality (Kim et al., 2010). These cultivars, selected because of their commercial importance in the Korean rice industry, were grown in the same paddy field at the National Institute of Crop Science, Rural Development Administration, Korea in 2010. They were manually hulled and ground to obtain a fine powder using a cyclone mixer mill (HMF-590; Hanil, Seoul, Korea) and a mortar and pestle. The milled rice powders were kept at �80 �C prior to extraction. All chemicals used in this study were of analytical grade. Methanol and chloroform, which were used as extraction solvents, were purchased from J.T. Baker (Phillipsburg, NJ, USA). Ribitol and N-methyl-N-trimethylsilyltrifluoroacetamide (MSTFA) were obtained from Sigma Chemical Co. (St. Louis, MO). Methoxyamine hydrochloride was purchased from Thermo Fisher Scientific (Pittsburgh, PA, USA).

Labs working on this Project

• National Academy of Agricultural Science, Rural Development Administration, Suwon 441-707, Republic of Korea

Corresponding Author

• S.-H. Ha: sunhwa@korea.kr