Os02g0510200

Bispyribac sodium (BS), a pyrimidinyl carboxy herbicide, is a well-known inhibitor of acetolactate synthase (ALS) activity. ALS is an enzyme in the biosynthetic pathway for branched-chain amino acids.

Annotated Information

Function

Bispyribac sodium (BS), a pyrimidinyl carboxy herbicide, is a well-known inhibitor of acetolactate synthase (ALS) activity. ALS is an enzyme in the biosynthetic pathway for branched-chain amino acids. BS can be used with OsmALS [W548L/S627I] to select transformed cells, and that this method is suitable for obtaining transgenic indica and indica-derived rice cultivars.

'Fig. 4 Colorimetric enzyme assay for ALS from non-transgenic and transgenic plants (T0 and T1 generation) of KL, TS, TA and NB. #21 and 24 of TS, #7 and 8 of TA, and #8 and 23 of NB are all T1 generations. BS- or BS? at the top of each reaction tube indicates pretreatment with or without 0.25 lM of BS, respectively. One nontransgenic plant and two transgenic plants were used for each cultivar.The distinct color reveals whether acetoin does (red) or does not(yellow) accumulate, indicating ALS activity is working or inhibited,respectively'

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