Os03g0216700

Please input one-sentence summary here.

Annotated Information

Mutation

• In this study, to examine the function and role of OsFRDL1 in rice, the researchers obtained two independent Tos-17 insertion lines; ND8025 (ND) and NC2637 (NC) for this gene. The OsFRDL1 was knocked out in the ND line, but knocked down in the NC line. When the wild-type rice and two Tos-17 insertion lines were grown at 10 mM Fe (as FeSO4), no visible difference was observed among the three lines (Fig. 3A). However, at 0.2 mM Fe, chlorosis was observed in the newly expanded leaves of two Tos-17 lines, but not in the wild type (Fig. 3B). The Fe3+ precipitation in the roots was investigated at 10 mM Fe with Perls blue staining (Green and Rogers, 2004). Fe precipitation was observed in the epidermal cells of the roots of all three lines (Fig. 3, C–E), which was not observed in Arabidopsis roots (Green and Rogers, 2004). This distinct precipitation in rice is attributed to secreted oxygen from rice roots, which oxidizes ferrous iron (Fe2+) into insoluble ferric iron (Fe3+) on the root surface (Horiguchi, 1995). However, there was no difference in the epidermal staining among three lines (Fig. 3, C–E). In contrast, heavy staining was observed in the central vascular part of the knockout line, ND (Fig. 3, D and G), but not in the wild-type line (Fig. 3, C and F). In the knockdown line NC, staining was also observed in the central vascular part (Fig. 3, E and H), although the intensity was not as strong as that of ND.

Figure 3. Phenotype of Tos-17 insertion lines of OsFRDL1. .

Labs working on this gene

• Research Institute for Bioresources, Okayama University, Kurashiki 710–0046, Japan