Os03g0296800
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Contents
Annotated Information
Function
Mutation
Expression Pattern
- Quantitative RT–PCR revealed that the transcripts of MIT were three times lower in the roots and shoots of plants exposed to Fe-limited conditions compared with plants grown hydroponically in the presence of 100 μM Fe. MIT transcripts were increased in the roots and shoots when the plants were exposed to excess Fe (500 μM Fe; Fig. 3a,b). To further understand its role during germination and seed development, the MIT promoter was used to drive the expression of β-glucuronidase in rice. MIT expression was observed during germination and at all stages of seed development. During germination, MIT expression was specific to the embryo (Fig. 3c–f). Expression was observed in the leaf primordia and coleorhizae 1 day after germination (Fig. 3d). It increased subsequently and, 3 days after germination, was observed in whole embryo (Fig. 3f). Expression was also observed from anthesis through seed development (Fig. 3g, left to right), supporting the hypothesis that MIT has a crucial role in these growth stages. Further the steady state transcripts of MIT were observed during all growth stages of rice plant including root, leaves, stem, anther, pistil, lemma, palea, ovary, embryo and endosperm as revealed by microarray analysis (Supplementary Fig. S1).
Expression analysis of MIT. (a, b) The changes in expression of MIT in response to Fe availability − Fe: 0 μM Fe; + Fe: 100 μM Fe; + + Fe: 500 μM Fe. (a) Root. (b) Shoot. (c–g) MIT promoter driven β-glucuronidase expression during germination (c–f) and seed development (g); (c) 0; (d) 1; (e) 2; (f) 3 days after germination. (g) From left to right, before anthesis 1, 4, 12, 16, 20 and 25 days after anthesis. Scale, 500 μm. The graph shows mean ± s.d.; *P < 0.05, ANO VA, n = 3. [1].
Evolution
Subcellular localization
Labs working on this gene
- Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-Ku, Tokyo 113-8657, Japan.
- Graduate School of Life Sciences, Toyo University, 1-1-1 Izumino Itakura-machi, Gunma 374-0193, Japan.
- Department of Plant Molecular Systems, Biotech and Crop Biotech Institute, Kyung Hee University, Yongin 446-701, Republic of Korea.
- Research Institute for Bioresources and Biotechnology, Ishikawa Prefectural University, Ishikawa 921-8836, Japan.
References
- ↑ Bashir K, Ishimaru Y, Shimo H, Nagasaka S, Fujimoto M, Takanashi H, Tsutsumi N, An G, Nakanishi H, Nishizawa NK. The rice mitochondrial iron transporter is essential for plant growth. Nat Commun. 2011;2:322. doi: 10.1038/ncomms1326. PubMed PMID: 21610725; PubMed Central PMCID: PMC3113228.
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