Os03g0427300

Glup4 gene encodes small GTPase, Rab5a in rice.

Annotated Information

Mutation

• Using RT-PCR in the presence of dUTPOregon

Green to label RNAs and rhodamine B hexyl ester to stain prolamine protein bodies, glutelin RNAs are found to be preferentially transported to the cisternal-ER (Cis-ER) in wildtype endosperm (Fig. 1). Prolamine RNAs, on the other hand, are targeted to ER that delimits the spherical prolamine protein bodies (PB-ER). In the glup4 mutant, however, glutelin RNAs are partially misdirected from the Cis-ER to the PB-ER (Fig. 1), as well as to large dilated multivesicular body-like structures located near the plasma membrane (data not shown). The glutelin RNA mistargeting was not only observed in one gene (Gt-2), but also in others (GluA-1, GluA- 3 and GluB-1, data not shown). Prolamine RNA localization to the PB-ER is unaffected (Fig. 1), suggesting that Rab5 is involved in glutelin RNA sorting only.

Fig. 1. Localization of prolamine and glutelin mRNAs in wildtype and glup4 developing rice endosperm. .

Labs working on this gene

• Institute of Biological Chemistry, Washington State University, Pullman WA, 99164-6340 USA
• Faculty of Agriculture, Kyushu University, 6-10-1 Hakozaki, Fukuoka 812-8581, Japan
• School of Molecular Biosciences, Washington State University, Pullman WA, 99164-7520 USA
• Present Address: International Liberal Arts Program, Akita International University, Akita 010-1292, Japan
• Present Address: Laboratory of Plant Molecular Genetics, Nara Institute of Science and Technology, 8916-5 Takayama, Ikoma, Nara 630-0101, Japan