Os12g0601400

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Annotated Information

Function

• The IAA gene, a well-studied gene in Arabidopsis that serves as a negative regulator of auxin signaling.
• OsIAA3 is one of these family members whose expression is rapidly increased in response to auxin.

Expression

• The researchers performed semiquantitative reverse transcriptase–polymerase chain reaction (RT-PCR) analysis using primers that specifically amplified the OsIAA3 3¢ non-coding region. Wild-type(WT) plants were treated with media containing 20 lM IAA. The OsIAA3 transcript was increased by IAA within 15–30 min,and reached a maximum within 1 h (Figure 2a).
• To confirm that OsIAA3 also interacts with Aux/IAA and ARF, we performed a yeast two-hybrid assay using OsIAA3 and

OsARF1 (Sato et al., 2001; Waller et al., 2002). All transformed yeast cells formed colonies on the medium with histidine, while only yeasts expressing both OsIAA3 protein and OsIAA3 or OsIAA3 and OsARF1 protein formed colonies on the medium without histidine (Figure 2b). This indicates that OsIAA3 interacts with both OsIAA and OsARF proteins,as do Aux/IAA proteins in Arabidopsis.

Evolution

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Labs working on this gene

• Bioscience and Biotechnology Center, Nagoya University, Chikusa, Nagoya, Aichi 464-8601, Japan

References

• Abel, S., Nguyen, M.D. and Theologis, A.(1995) The PS-IAA4/5-like family of early auxin-inducible messenger-RNAs in Arabidopsis thaliana. J. Mol. Biol. 251, 533–549.