Os01g0323600

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OsSAMS1 was expressed abundantly in almost every tissue. OsSAMS2 was preferentially expressed in young roots and panicles. By contrast, the expression of OsSAMS3 was weak compared with OsSAMS1 (Fig. 1A).Thus, OsSAMS genes were constitutively expressed but the various homologues may have different functions during development.The double mutant containing OsSAMS1 or 2, or 3 alone no longer depended on externally added SAM when their expression was induced in the presence of galactose (Fig. 1B). Thus, OsSAMS1, 2 and 3 were all genetically complemented for the functional SAMS enzyme in the yeast double mutant in vivo. Moreover, as shown in Fig. 1B, the OsSAMS1 activity was comparable to OsSAMS2, and OsSAMS3 activity was weak.

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Real-time RT-PCR analysis of OsSAMS1 revealed reduced expression in the transgenic lines W, M and S, by 60.4%, 4.8%, 2.6% that of the WT, respectively, and OsSAMS2 and OsSAMS3 also showed a significant decrease in expression levels (Fig. 3A). The expression of OsSAMS2 was decreased to 76.1%, 31.5% and 15.9% in the W, M and S lines.

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Research Center for Molecular and Developmental Biology, Key Laboratory of Photosynthesis and Environmental Molecular Physiology, Institute of Botany, Chinese Academy of Sciences, Beijing 100093, China
Graduate School of the Chinese Academy of Sciences, Beijing 100049, China
National Plant Gene Research Center, Beijing 100093, China
Key Laboratory of Molecular Biology, College of Heilongjiang Province, Heilongjiang University, Harbin 150080, China

Baurle I, Dean C. The timing of developmental transitions in plants. Cell 2006;125:655–64.