Os03g0737701

lpa N15-186 is a mutant allele of the rice myo-inositol kinase (OsMIK) gene.

Annotated Information

Function

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Mutation

• In order to conWrm that these two mutants are lpa mutants, the seed total P, phytic acid-P (PA-P) and inorganic P (Pi) contents were examined (Table 1).

Table 1 Comparison of seed myo-inositol, phytic acid and inorganic P contents of lpa mutants and wild-types. .

• Seed extracts of lpa mutants were subjected to HPLC analysis to determine if the other inositol phosphates were aVected by the mutation. Comparison to wild-type Nipponbare and inositol phosphate standards revealed that N15- 186 seeds are signiWcantly reduced in PA and inositol monophosphate while exhibiting an increase in Pi (Fig. 1). No signiWcant accumulation of other inositol phosphate intermediates (InsP2 to InsP5) was detected under the conditions used.

[[File:Os03g0737701-2.png|center|thumb|400px|Fig. 1 HPLC chromatograms of inositol phosphates. a Inositol phosphate standards, retention times for the various inositol phosphates were: Ins(2)P1, 9.5 min; Pi, 15.5 min, Ins(1,4)P2, 21 min; Ins(1,3,4)P3, 32 min; Ins(13,4,5)P4, 33 min; Ins(1,3,4,5,6)P5, 35 min; and Ins(1,2,3,4,5,6)P6, 29 min. b Nipponbare wild-type, c N15-186 lpa mutant. .]

• The GC–MS analysis revealed that the myo-inositol content,

as well as those of other sugars (glucose, fructose and galactose), was increased in the N15-186 mutant compared to the wild-type (Fig. 2).

[[File:Os03g0737701-3.png|center|thumb|400px|Fig. 2 GC–MS analysis of myoinositol and other carbohydrates as alditol hexaacetate derivatives from seed extracts of wild-type, N-186 and N-375 mutants. .]

Expression

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Evolution

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Labs working on this gene

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