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Figure 4. In situ hybridization analyses of OsRad21-4 mRNA in flowers. Traverse sections of flowers in premeiotic PMC stage (A and B), meiotic PMC stage (C). or uninucleate pollen stage (D) were hybridized with DIG-labeled antisense RNA (A, C and D) or sense RNA (B, control) probe. Signals were detected with an anti-DIG-antibody-conjugated alkaline phosphatase and visualized with the chromagenic substrates NBT and BCIP (Roche). PMCs, pollen mother cells; T, tapetum; P, uninucleate pollen. Bars in (A) = 20 lm for (A)–(C), and in (D)= 20 lm

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current15:34, 20 May 2014Thumbnail for version as of 15:34, 20 May 2014827 × 649 (107 KB)Mmlin66 (talk | contribs)Figure 4. In situ hybridization analyses of OsRad21-4 mRNA in flowers. Traverse sections of flowers in premeiotic PMC stage (A and B), meiotic PMC stage (C). or uninucleate pollen stage (D) were hybridized with DIG-labeled antisense RNA (A, C and D) or
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