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Figure 4 GW5 expression analyses. (A) The sequence of the predicted ORF2. The primers (F6, R2 and R3) used for RT-PCR analysis and the predicted NLS are denoted by arrows and a line, respectively. In addition, amino-acid residues 20-71 formed an arginine-rich region. (B) RT-PCR analysis of ORF2 using mRNA derived from the young panicle of CSSL28. Lane 1: molecular marker. Lane 2: a 140-bp RT-PCR product using primers F6 and R3 (marker 1). Lane 3: a 200-bp RT-PCR product using primers F6 and R2 (marker 02). Lane 4: control (no template was added in the PCR reaction).
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| Date/Time | Thumbnail | Dimensions | User | Comment | |
|---|---|---|---|---|---|
| current | 13:50, 8 June 2014 | | 501 × 514 (187 KB) | Mafeifei (talk | contribs) | Figure 4 GW5 expression analyses. (A) The sequence of the predicted ORF2. The primers (F6, R2 and R3) used for RT-PCR analysis and the predicted NLS are denoted by arrows and a line, respectively. In addition, amino-acid residues 20-71 formed an arginine- |
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