File:OsPDR91.jpg

Fig. 2. Early ospdr9 expression in rice roots in response to various stresses. RT-PCR products of ospdr9 and rac1 (upper panels) and adh1 and rac1 (lower panels) were separated on 1.1% agarose gels and stained with ethidium bromide.3RT lanes show typical 3RT reactions in which reverse transcriptase was omitted. gDNA lanes show PCR amplifications on genomic DNA with each primer pair in the same conditions. Sizes of cDNA (left) or gDNA products (right) are indicated. A: RNA was prepared from the roots of rice seedlings that were incubated on growth medium (controls C1,C2 and C3), incubated on growth medium supplemented with PEG (15 or 13.5%), kept in the cold (4oC, CO), exposed to heat (42oC, H), or incubated on growth medium supplemented with ZnSO4 (5 or 2.5 mM) for 2 h. B: Rice seedlings were incubated on growth medium (controls C4 and C5) or on growth medium supplemented with KCl (5 mM), MgSO4 (2.5 mM), CoCl2 (250 WM and 2.5 mM), NiCl2 (250 WM and 2.5 mM), or ethanol (0.5% and 5%) for 2 h. Equal amounts of rac1 were detected, except during ethanol toxicity (5%) which caused a decline of the rac1 messenger.