Os09g0547500
The rice Os09g0547500 was reported as OsLDC-like 1 in 2012 [1] by researchers from Korea.
Contents
Annotated Information
Gene Symbol
- Os09g0547500 <=> OsLDC-like 1,ABCC1,OsMRP1,MRP1
Function
- The rice OsLDC-like 1 encodes a lysine decarboxylase-like protein.
Phenotypic analysis
- The mutant line exhibited enhanced accumulation of the polyamines (PAs) putrescine, spermidine, and spermine under conditions of oxidative stress.
- The generation of reactive oxygen species (ROS) in the mutant line was assessed by qRT-PCR analysis of NADPH oxidase (RbohD and RbohF), and by DCFH-DA staining.
- Cellular levels of ROS in osldc-like 1 leaves were significantly lower than those in the wild-type (WT) rice after exposure to oxidative, high salt and acid stresses. Exogenously-applied PAs such as spermidine and spermine significantly inhibited the stress-induced accumulation of ROS and cell damage in WT leaves.
- Additionally, the activities of ROS-detoxifying enzymes were increased in the homozygous mutant line in the presence or absence of H2O2.
- Thus, mutation of OsLDC-like 1 conferred an oxidative stress-tolerant phenotype.
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Labs working on this gene
- Department of Biology, Sunchon National University, Sunchon 540-742, Korea
- Crop Biotech Institute Center, Department of Plant Systems Biotech, Kyung Hee University, Yongin 446-701, Korea
References
- ↑ Jang, Su Jin, et al. "Increased polyamine biosynthesis enhances stress tolerance by preventing the accumulation of reactive oxygen species: T-DNA mutational analysis of Oryza sativa lysine decarboxylase-like protein 1." Molecules and cells 34.3 (2012): 251-262.
