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Date Name Thumbnail Size Description Versions
15:49, 6 June 2014 Os01g08415008.jpg (file) 39 KB Figure 8. Cell cycle progression response to cold in flow cytometry assay in the OsMYB3R-2 transgenic lines. A, The wild type at 28℃. B, The wild type at 4℃. C, Overexpressing line 7 (OL7) of OsMYB3R-2 transgenic rice at 28℃. D, Overexpressing line 1
15:48, 6 June 2014 Os01g08415007.jpg (file) 29 KB Figure 7. Expression patterns of cyclin genes in the OsMYB3R-2 transgenic rice, cold response and survival ratio, and cellular free Pro in the OsCycB1;1 transgenic rice lines. A, Expression patterns of cyclin-responsive genes in wild-type (WT) and OsMYB3R 1
15:47, 6 June 2014 Os01g08415006.jpg (file) 74 KB Figure 6. DNA binding affinity of OsMYB3R-2 protein. A, The alignment of MSA-like sequences shown in the promoters of type B cyclin genes: rice OsCycB1;1 and OsKNOLLE2, Arabidopsis cyc1bAt (Day et al., 1996) and cyc2aAt (Ferreira et al., 1994), and tobacc 1
15:46, 6 June 2014 Os01g08415005.jpg (file) 89 KB Figure 5. Transcription activation analysis of OsMYB3R-2 protein. A, Different pGBKT7-OsMYB3R-2 vector constructs. The truncated cDNA fragments of OsMYB3R-2 were sequenced and inserted into the NdeI-PstI sites, with ATG added at the end of the NdeI site i 1
15:46, 6 June 2014 Os01g08415004.jpg (file) 37 KB Figure 4. Gene expression patterns and free Pro levels of transgenic rice plants in response to cold. A, Expression pattern of OsCPT1 in wild-type and OsMYB3R-2 transgenic rice at room temperature (25℃) or low temperature (4℃). Tubulin was used as an 1
15:44, 6 June 2014 Os01g08415003.jpg (file) 68 KB Figure 3. Tolerance response of the OsMYB3R-2 transgenic lines to cold stress. A, Wild-type and OsMYB3R-2 transgenic 2-week-old rice seedlings at the same stage before the 2℃ treatment. B, Seedlings were grown in the greenhouse for 2 weeks after 2℃ tr 1
15:40, 6 June 2014 Os01g08415002.jpg (file) 60 KB Figure 2. Phenotype analysis of the T2 generation of OsMYB3R-2 transgenic rice. A, The germination and growth of OsMYB3R-2 transgenic rice at 30℃. Shelled seeds of rice at 0 d; germinating seeds at 1, 2, and 3 d; young seedlings transferred to light (12 1
15:34, 6 June 2014 Os01g08415001.jpg (file) 52 KB Figure 1. Identification of OsMYB3R-2 transgenic rice and its expression pattern. A, Northern-blot assay of rice transgenic plants. Total RNA isolated from wild-type (WT) or transformed plants underwent hybridization with a [a-32P]dCTP-labeled probe of Os 1
03:05, 30 May 2014 Figure 8.jpg (file) 39 KB Figure 8. Cell cycle progression response to cold in flow cytometry assay in the OsMYB3R-2 transgenic lines. A, The wild type at 28℃. B, The wild type at 4℃. C, Overexpressing line 7 (OL7) of OsMYB3R-2 transgenic rice at 28℃. D, Overexpressing line 1
03:04, 30 May 2014 Figure 7.jpg (file) 29 KB Figure 7. Expression patterns of cyclin genes in the OsMYB3R-2 transgenic rice, cold response and survival ratio, and cellular free Pro in the OsCycB1;1 transgenic rice lines. A, Expression patterns of cyclin-responsive genes in wild-type (WT) and OsMYB3R 2
03:03, 30 May 2014 Figure 6.jpg (file) 74 KB Figure 6. DNA binding affinity of OsMYB3R-2 protein. A, The alignment of MSA-like sequences shown in the promoters of type B cyclin genes: rice OsCycB1;1 and OsKNOLLE2, Arabidopsis cyc1bAt (Day et al., 1996) and cyc2aAt (Ferreira et al., 1994), and tobacc 2
03:02, 30 May 2014 Figure 4.jpg (file) 37 KB Figure 4. Gene expression patterns and free Pro levels of transgenic rice plants in response to cold. A, Expression pattern of OsCPT1 in wild-type and OsMYB3R-2 transgenic rice at room temperature (25℃) or low temperature (4℃). Tubulin was used as an 2