| URL: | http://biodb.swu.edu.cn/qprimerdb |
| Full name: | real-time quantitative PCR Primer Database |
| Description: | The qPrimerDB database (real-time quantitative PCR Primer Database, http://biodb.swu.edu.cn/qprimerdb) is the most comprehensive qPCR primer database available to date, with a web front-end providing gene-specific and pre-computed primer pairs across 212 important organisms, including human, mouse, zebrafish, yeast, thale cress, rice, and maize. |
| Year founded: | 2018 |
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| Country/Region: | China |
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| University/Institution: | Southwest University |
| Address: | Academy of Agricultural Sciences, Southwest University, Beibei, Chongqing 400715, China |
| City: | Chongqing |
| Province/State: | Chongqing |
| Country/Region: | China |
| Contact name (PI/Team): | Kun Lu |
| Contact email (PI/Helpdesk): | drlukub@swu.edu.cn |
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qPrimerDB: A Powerful and User-Friendly Database for qPCR Primer Design. [PMID: 34773623]
Real-time quantitative polymerase chain reaction (qPCR) is a powerful tool for analyzing and quantifying gene expression, and its primer design is the first and most important step. In order to improve the efficiency and effectiveness of primer design, we designed a database qPrimerDB, based on thermodynamics gene-specific for multispecies qPCR primers design.In this chapter, we explained the working principle of the database and detailed the step-by-step practical steps with examples. The valuable and time-saving qPrimerDB database is publicly accessible at http://biodb.swu.edu.cn/qprimerdb and will be routinely updated. |
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qPrimerDB: a thermodynamics-based gene-specific qPCR primer database for 147 organisms. [PMID: 28977518]
Real-time quantitative polymerase chain reaction (qPCR) is one of the most important methods for analyzing the expression patterns of target genes. However, successful qPCR experiments rely heavily on the use of high-quality primers. Various qPCR primer databases have been developed to address this issue, but these databases target only a few important organisms. Here, we developed the qPrimerDB database, founded on an automatic gene-specific qPCR primer design and thermodynamics-based validation workflow. The qPrimerDB database is the most comprehensive qPCR primer database available to date, with a web front-end providing gene-specific and pre-computed primer pairs across 147 important organisms, including human, mouse, zebrafish, yeast, thale cress, rice and maize. In this database, we provide 3331426 of the best primer pairs for each gene, based on primer pair coverage, as well as 47760359 alternative gene-specific primer pairs, which can be conveniently batch downloaded. The specificity and efficiency was validated for qPCR primer pairs for 66 randomly selected genes, in six different organisms, through qPCR assays and gel electrophoresis. The qPrimerDB database represents a valuable, timesaving resource for gene expression analysis. This resource, which will be routinely updated, is publically accessible at http://biodb.swu.edu.cn/qprimerdb. |