Gene Expression Nebulas
A data portal of transcriptomic profiles analyzed by a unified pipeline across multiple species

Gene Expression Nebulas

A data portal of transcriptome profiles across multiple species

PRJCA000233: RNA-seq of 2 maize RILs under drought stress

Source: NGDC / CRA002420
Submission Date: May 14 2016
Release Date: May 14 2016
Update Date: -

Summary: -

Overall Design: Transcriptome analysis of 2 maize recombination inbred lines from ph4CV and F9721 under drought stress. The three-leaf seedlings of the two RILs were treated with natural drought stress without water. From the start to 5 days after drought stress, the shoot tissues of the two RILs seedlings were harvested and samples labeled CK, DT1, DT2 and DT5 were used for RNA extraction and Illumina deep sequencing.

GEN Datasets:
GEND000309
Strategy:
Species:
Tissue:
Development Stage:
Protocol
Growth Protocol: -
Treatment Protocol: Transcriptome analysis of 2 maize recombination inbred lines from ph4CV and F9721 under drought stress. The three-leaf seedlings of the two RILs were treated with natural drought stress without water.
Extract Protocol: -
Library Construction Protocol: -
Sequencing
Molecule Type: poly(A)+ RNA
Library Source:
Library Layout: PAIRED
Library Strand: -
Platform: ILLUMINA
Instrument Model: Illumina HiSeq 2500; Illumina Genome Analyzer II
Strand-Specific: Unspecific
Samples
Basic Information:
Sample Characteristic:
Biological Condition:
Experimental Variables:
Protocol:
Sequencing:
Assessing Quality:
Analysis:
Data Resource GEN Sample ID GEN Dataset ID Project ID BioProject ID Sample ID Sample Name BioSample ID Sample Accession Experiment Accession Release Date Submission Date Update Date Species Race Ethnicity Age Age Unit Gender Source Name Tissue Cell Type Cell Subtype Cell Line Disease Disease State Development Stage Mutation Phenotype Case Detail Control Detail Growth Protocol Treatment Protocol Extract Protocol Library Construction Protocol Molecule Type Library Layout Strand-Specific Library Strand Spike-In Strategy Platform Instrument Model Cell Number Reads Number Gbases AvgSpotLen1 AvgSpotLen2 Uniq Mapping Rate Multiple Mapping Rate Coverage Rate
Publications
ZmbHLH124 identified in maize recombinant inbred lines contributes to drought tolerance in crops.
Plant biotechnology journal . 2021-05-25 [PMID: 34031958]