Summary: Exploring regulatory mechanism of rice tiller angle through dynamic transcriptome of shoot gravitropism
Strategy: |
|
Species: |
|
Tissue: |
|
Height/Length/Weight: |
|
Isolation_source: |
|
Growth Protocol: | For RNA-seq and time-lapse photography, germinated ZH11 seeds were grown on 0.4% (w/v) agar plates at 28°C for 3 d under a 16-h-light/8-h-dark photoperiod, and then seedlings with uniform growth were transferred into soil and grown under controlled greenhouse conditions (16 h light and 8 h dark at 28°C, 150-200 μmol photons m-2 s-1) for 11 d prior to the gravistimulation treatments. |
Treatment Protocol: | Shoot bases (1.5 cm in length) were harvested at 12 time points (0, 0.25, 0.5, 1, 1.5, 2, 3, 4, 5, 6, 7, and 8 h) after the start of gravistimulation and were frozen immediately in liquid nitrogen. |
Extract Protocol: | RNA was extracted using a TRIzol kit according to the user manual (Invitrogen). |
Library Construction Protocol: | Illumina Part #15026495 Rev.D |
Molecule Type: | poly(A)+ RNA |
Library Source: | |
Library Layout: | Single |
Library Strand: | - |
Platform: | ILLUMINA |
Instrument Model: | Illumina HiSeq 2000 |
Strand-Specific: | Unspecific |
Data Resource | GEN Sample ID | GEN Dataset ID | Project ID | BioProject ID | Sample ID | Sample Name | BioSample ID | Sample Accession | Experiment Accession | Release Date | Submission Date | Update Date | Species | Race | Ethnicity | Age | Age Unit | Gender | Source Name | Tissue | Cell Type | Cell Subtype | Cell Line | Disease | Disease State | Development Stage | Mutation | Phenotype | Case Detail | Control Detail | Growth Protocol | Treatment Protocol | Extract Protocol | Library Construction Protocol | Molecule Type | Library Layout | Strand-Specific | Library Strand | Spike-In | Strategy | Platform | Instrument Model | Cell Number | Reads Number | Gbases | AvgSpotLen1 | AvgSpotLen2 | Uniq Mapping Rate | Multiple Mapping Rate | Coverage Rate |
---|