Gene Expression Nebulas
A data portal of transcriptomic profiles analyzed by a unified pipeline across multiple species

Gene Expression Nebulas

A data portal of transcriptome profiles across multiple species

PRJCA002591: Single cell transcriptome analysis reveals six subpopulations reflecting distinct cellular fates in senescent mouse embryonic fibroblasts

Source: NGDC / CRA002582
Submission Date: Api 24 2020
Release Date: Apr 24 2020
Update Date: Api 24 2020

Summary: We conducted single cell RNA sequencing on senescent MEFs. PCA analyses showed there were obvious heterogeneity among these MEFs and they could be divided into six subpopulations. Three lines of gene expression analyses indicated distinct expression features of these six subpopulations.

GEN Datasets:
GEND000173
Strategy:
Species:
Tissue:
Cell Type:
Protocol
Growth Protocol: -
Treatment Protocol: -
Extract Protocol: -
Library Construction Protocol: Following the manual SMART-Seq v4 Ultra Low Input RNA Kit for the Fluidigm C1 System, IFCs Components
Sequencing
Molecule Type: poly(A)+ RNA
Library Source:
Library Layout: PAIRED
Library Strand: -
Platform: ILLUMINA
Instrument Model: Illumina HiSeq 4000
Strand-Specific: Unspecific
Samples
Basic Information:
Sample Characteristic:
Biological Condition:
Experimental Variables:
Protocol:
Sequencing:
Assessing Quality:
Analysis:
Data Resource GEN Sample ID GEN Dataset ID Project ID BioProject ID Sample ID Sample Name BioSample ID Sample Accession Experiment Accession Release Date Submission Date Update Date Species Race Ethnicity Age Age Unit Gender Source Name Tissue Cell Type Cell Subtype Cell Line Disease Disease State Development Stage Mutation Phenotype Case Detail Control Detail Growth Protocol Treatment Protocol Extract Protocol Library Construction Protocol Molecule Type Library Layout Strand-Specific Library Strand Spike-In Strategy Platform Instrument Model Cell Number Reads Number Gbases AvgSpotLen1 AvgSpotLen2 Uniq Mapping Rate Multiple Mapping Rate Coverage Rate
Publications
Single-Cell Transcriptome Analysis Reveals Six Subpopulations Reflecting Distinct Cellular Fates in Senescent Mouse Embryonic Fibroblasts.
Frontiers in genetics . 2020-08-11 [PMID: 32849838]