Summary: We conducted single cell RNA sequencing on senescent MEFs. PCA analyses showed there were obvious heterogeneity among these MEFs and they could be divided into six subpopulations. Three lines of gene expression analyses indicated distinct expression features of these six subpopulations.
Strategy: |
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Species: |
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Tissue: |
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Cell Type: |
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Growth Protocol: | - |
Treatment Protocol: | - |
Extract Protocol: | - |
Library Construction Protocol: | Following the manual SMART-Seq v4 Ultra Low Input RNA Kit for the Fluidigm C1 System, IFCs Components |
Molecule Type: | poly(A)+ RNA |
Library Source: | |
Library Layout: | PAIRED |
Library Strand: | - |
Platform: | ILLUMINA |
Instrument Model: | Illumina HiSeq 4000 |
Strand-Specific: | Unspecific |
Data Resource | GEN Sample ID | GEN Dataset ID | Project ID | BioProject ID | Sample ID | Sample Name | BioSample ID | Sample Accession | Experiment Accession | Release Date | Submission Date | Update Date | Species | Race | Ethnicity | Age | Age Unit | Gender | Source Name | Tissue | Cell Type | Cell Subtype | Cell Line | Disease | Disease State | Development Stage | Mutation | Phenotype | Case Detail | Control Detail | Growth Protocol | Treatment Protocol | Extract Protocol | Library Construction Protocol | Molecule Type | Library Layout | Strand-Specific | Library Strand | Spike-In | Strategy | Platform | Instrument Model | Cell Number | Reads Number | Gbases | AvgSpotLen1 | AvgSpotLen2 | Uniq Mapping Rate | Multiple Mapping Rate | Coverage Rate |
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