Gene Expression
Nebulas
Gene Expression NebulasSummary: We report the expression of selected genes associated with cortical, striatal and olfactory interneurons in hSS-derived Dlxi1/2::eGFP cells before (in hSS) and after migration (in hCS). By fusing hiPSC-derived fusions that were separately patterned to generate either human subpallial spheroids (hSS) or human cortical spheroids (hCS) we are able to model human interneuron migration in vitro. The aim of this experiment is to examine the gene expression of hSS-derived Dlxi1/2::eGFP cells before and after migration.
Overall Design: Examination of Dlxi1/2::eGFP cells in hSS (not migrated) and Dlxi1/2::eGFP cells in hCS (migrated).
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| Growth Protocol: | Differentiation of human iPSC into subpallium-like spheroids; 3D cultures were dissociated and FACS was used to isolate eGFP cells |
| Treatment Protocol: | - |
| Extract Protocol: | The single-cell RNA-Seq library preparation was adapted from the original SMART Seq-2 protocol. Single-cells were FACS sorted into a 96-well PCR plate followed by lysis, reverse transcription and PCR amplification according to SMART-seq2 protocol. |
| Library Construction Protocol: | After checking the quality control of cDNA library, final libraries were prepared using Nextera XT library prep kit (96 index primers, Illumina) protocol |
| Molecule Type: | poly(A)+ RNA |
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| Library Layout: | SINGLE |
| Library Strand: | - |
| Platform: | ILLUMINA |
| Instrument Model: | Illumina HiSeq 2500 |
| Strand-Specific: | Unspecific |
| Data Resource | GEN Sample ID | GEN Dataset ID | Project ID | BioProject ID | Sample ID | Sample Name | BioSample ID | Sample Accession | Experiment Accession | Release Date | Submission Date | Update Date | Species | Race | Ethnicity | Age | Age Unit | Gender | Source Name | Tissue | Cell Type | Cell Subtype | Cell Line | Disease | Disease State | Development Stage | Mutation | Phenotype | Case Detail | Control Detail | Growth Protocol | Treatment Protocol | Extract Protocol | Library Construction Protocol | Molecule Type | Library Layout | Strand-Specific | Library Strand | Spike-In | Strategy | Platform | Instrument Model | Cell Number | Reads Number | Gbases | AvgSpotLen1 | AvgSpotLen2 | Uniq Mapping Rate | Multiple Mapping Rate | Coverage Rate |
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