Gene Expression
Nebulas
Gene Expression NebulasSummary: Diminishing potential to replace damaged tissues is a hallmark for ageing of somatic stem cells, but the mechanisms leading to ageing remain elusive. We present a proteome-wide atlas of age-associated alterations in human haematopoietic stem and progenitor cells (HPCs) along with five other cell types that constitute the bone marrow niche. For each, the abundance of a large fraction of the ~12,000 proteins identified was assessed in a cohort of healthy human subjects from different age. As the HPCs became older, pathways in central carbon metabolism exhibited features reminiscent of the Warburg effect where glycolytic intermediates are rerouted towards anabolism. Simultaneously, altered abundance of early regulators of HPC differentiation revealed a reduced functionality and a bias towards myeloid differentiation at the expense of lymphoid development. Ageing caused significant alterations in the bone marrow niche too, such as functionality of the pathways involved in HPC homing and lineage differentiation. The data represents a valuable resource for further in-depth mechanistic analyses, and for validation of knowledge gained from animal models.
Overall Design: Examination of myeloid- and lymphoid-primed sub-populations of HPCs across young and old donors
| Strategy: |
|
| Species: |
|
| Tissue: |
|
| Healthy Condition: |
|
| Development Stage: |
|
| Growth Protocol: | Bone marrow (BM) samples were harvested through puncture at the posterior iliac crest using a Yamshidi needle |
| Treatment Protocol: | BM aspirates were processed by FICOLL density fractionation for isolation of MNCs; stained with CD34-APC, CD45-FITC and CD14-PE; FACS-sorted and stored at -80C |
| Extract Protocol: | Sequencing libraries from 192 single CD34+ cells per donor were produced based on the smart-seq2 protocol of Picelli et al. 2014 and the tagmentation procedure of Henning et al. 2018 with slight modifications as described in the publication. |
| Library Construction Protocol: | - |
| Molecule Type: | rRNA- RNA |
| Library Source: | |
| Library Layout: | SINGLE |
| Library Strand: | -; Reverse; Forward |
| Platform: | ILLUMINA |
| Instrument Model: | Illumina NextSeq 500 |
| Strand-Specific: | Unspecific; Specific |