Summary: We report the data generated upon knockdown of TCF4 in hiPSC-derived neural progenitor cells and glutamatergic neurons.
Overall Design: Examination of TCF4 knockdown to probe its contribution to schizophrenia.
Strategy: |
|
Species: |
|
Tissue: |
|
Healthy Condition: |
|
Cell Type: |
|
Cell Line: |
|
Development Stage: |
|
Growth Protocol: | The hiPSC line used for deriving neurons was generated by Sendai virus method at Rutgers University Cell and DNA Repository (RUCDR)-NIMH Stem Cell Center from cryopreserved lymphocytes (CPLs) of a 29-year-old male SCZ patient (cell ID: 07C65853) from the MGS collection. The NorthShore University HealthSystem Institutional Review Board (IRB) approved the study. hiPSCs were cultured using the feeder-free method in Geltrex (Thermofisher)-coated plates in mTeSR1 medium (StemCell). The media were changed daily, and cell were passaged as clumps every 5 days using ReLeSR (StemCell) in the presence of 5 μm ROCK inhibitor (R&D Systems). hiPSCs were characterized by positive IF staining of pluripotent stem cell markers OCT4, TRA-1-60, NANOG, and SSEA4. |
Treatment Protocol: | - |
Extract Protocol: | Total RNAs were extracted from homogenized cell lysate using the RNeasy Plus mini kit (Qiagen) |
Library Construction Protocol: | RNA libraries were prepared for sequencing using standard Illumina protocols. |
Molecule Type: | rRNA- RNA |
Library Source: | |
Library Layout: | PAIRED |
Library Strand: | - |
Platform: | ILLUMINA |
Instrument Model: | Illumina HiSeq 2500 |
Strand-Specific: | Unspecific |
Data Resource | GEN Sample ID | GEN Dataset ID | Project ID | BioProject ID | Sample ID | Sample Name | BioSample ID | Sample Accession | Experiment Accession | Release Date | Submission Date | Update Date | Species | Race | Ethnicity | Age | Age Unit | Gender | Source Name | Tissue | Cell Type | Cell Subtype | Cell Line | Disease | Disease State | Development Stage | Mutation | Phenotype | Case Detail | Control Detail | Growth Protocol | Treatment Protocol | Extract Protocol | Library Construction Protocol | Molecule Type | Library Layout | Strand-Specific | Library Strand | Spike-In | Strategy | Platform | Instrument Model | Cell Number | Reads Number | Gbases | AvgSpotLen1 | AvgSpotLen2 | Uniq Mapping Rate | Multiple Mapping Rate | Coverage Rate |
---|