Growth Protocol:	MSCs from bone marrow Ewing patients were isolated by density-gradient centrifugation using Ficoll technique and were cultured in alpha MEM (Gibco), MSC-qualified serum (Gibco), 1% L-glutamine (Gibco) and 1 ng/mL bFGF (Sigma), added ex-tempo. CLB-berlud are cultured in RPMI (Gibco) 10% FBS (Eurobio), 100U/mL.
Treatment Protocol:	No treatment
Extract Protocol:	Dissociated cells were captured and processed with the C1 Single-Cell Auto Prep System (FluidigmTM) following the manufacturer' protocol
Library Construction Protocol:	After harvest from the C1 device, each cDNA library was tagmented using the Nextera XT DNA Sample Preparation Kit (Illumina). Libraries were quantified by Qubit fluorometric assay (Invitrogen) with dsDNA HS (High Sensitivity) Assay Kit and qualified using LabChip (LabChip® GX Touch™ PerkinElmer)

Molecule Type:	poly(A)+ RNA
Library Source:
Library Layout:	paired
Library Strand:	-
Platform:	Illumina
Instrument Model:	Illumina HiSeq 2500
Strand-Specific:	Unspecific

Data Resource	GEN Sample ID	GEN Dataset ID	Project ID	BioProject ID	Sample ID	Sample Name	BioSample ID	Sample Accession	Experiment Accession	Release Date	Submission Date	Update Date	Species	Race	Ethnicity	Age	Age Unit	Gender	Source Name	Tissue	Cell Type	Cell Subtype	Cell Line	Disease	Disease State	Development Stage	Mutation	Phenotype	Case Detail	Control Detail	Growth Protocol	Treatment Protocol	Extract Protocol	Library Construction Protocol	Molecule Type	Library Layout	Strand-Specific	Library Strand	Spike-In	Strategy	Platform	Instrument Model	Cell Number	Reads Number	Gbases	AvgSpotLen1	AvgSpotLen2	Uniq Mapping Rate	Multiple Mapping Rate	Coverage Rate