Gene Expression Nebulas
A data portal of transcriptomic profiles analyzed by a unified pipeline across multiple species

Gene Expression Nebulas

A data portal of transcriptome profiles across multiple species

PRJNA533609: Single cell RNA-Seq of Neuroblastoma cell line (CLB-berlud)

Source: NCBI / GSE130022
Submission Date: Apr 18 2019
Release Date: Feb 14 2020
Update Date: Feb 14 2020

Summary: We report the application of single-molecule-based sequencing technology for high-throughput profiling of Neuroblastoma cell line (CLB-berlud) Examination of gene expression in Neuroblastoma cell line (CLB-berlud), as a negative control

Overall Design: Study of transcriptomic heterogeneity of Ewing Sarcoma models and comparison with control cell models

GEN Datasets:
GEND000064
Strategy:
Species:
Healthy Condition:
Cell Type:
Cell Line:
Protocol
Growth Protocol: CLB-berlud are cultured in RPMI (Gibco) 10% FBS (Eurobio), 100U/mL
Treatment Protocol: No treatment
Extract Protocol: Dissociated cells were captured and processed with the C1 Single-Cell Auto Prep System (FluidigmTM) following the manufacturer' protocol
Library Construction Protocol: After harvest from the C1 device, each cDNA library was tagmented using the Nextera XT DNA Sample Preparation Kit (Illumina). Libraries were quantified by Qubit fluorometric assay (Invitrogen) with dsDNA HS (High Sensitivity) Assay Kit and qualified using LabChip(LabChip® GX Touch™ PerkinElmer)
Sequencing
Molecule Type: poly(A)+ RNA
Library Source:
Library Layout: paired
Library Strand: -
Platform: Illumina
Instrument Model: Illumina HiSeq 2500
Strand-Specific: Unspecific
Samples
Basic Information:
Sample Characteristic:
Biological Condition:
Experimental Variables:
Protocol:
Sequencing:
Assessing Quality:
Analysis:
Data Resource GEN Sample ID GEN Dataset ID Project ID BioProject ID Sample ID Sample Name BioSample ID Sample Accession Experiment Accession Release Date Submission Date Update Date Species Race Ethnicity Age Age Unit Gender Source Name Tissue Cell Type Cell Subtype Cell Line Disease Disease State Development Stage Mutation Phenotype Case Detail Control Detail Growth Protocol Treatment Protocol Extract Protocol Library Construction Protocol Molecule Type Library Layout Strand-Specific Library Strand Spike-In Strategy Platform Instrument Model Cell Number Reads Number Gbases AvgSpotLen1 AvgSpotLen2 Uniq Mapping Rate Multiple Mapping Rate Coverage Rate
Publications
Transcriptional Programs Define Intratumoral Heterogeneity of Ewing Sarcoma at Single-Cell Resolution.
Cell reports . 2020-02-01 [PMID: 32049009]