Growth Protocol:	Cells were grown in rich media with either glucose (YP-glucose-5%) or maltose (YP-maltose-10%) in turbido-stat conditions. The cell counts were kept below 10 million cells per ml.
Treatment Protocol:	The cells were initially inoculated in YP-maltose-10% and grown in turbido-stat for 3 overnights. Afterwards they were washed into glucose media (3x, 5min, 3000rpm). After 6h of growth in glucose, the first sample was taken (glu_6h). After 12h of glucose growth the 2nd sample was taken (glu_12h). Afterwards the cells were washed into maltose media, were they cease to grow and enter lag phase. 1h after the shift to maltose the 3rd sample was taken (in_lag_1h). After 3h in lag phase the 4th sample was taken (in_lag_3h). The 5th sample is a 50-50 mix of cells from two growth stages: A part of it is the cells from maltose pregrowth, and another part is the cells from glu_12h sample.
Extract Protocol:	We used the Chromium Single Cell 3′ kit (v2) of 10x Genomics for scRNA-seq. We modified the protocol to include zymolyase for digestion of the cell wall. The cells were pre-grown in 3 mL maltose media (YPMAL10%) for two overnights in dilute conditions. The media was refreshed after the first overnight and at each step serial dilutions were made to capture cultures with a cell count of ~2*106 cells/mL. At the end of maltose pre-growth, the cells were washed and inoculated into 50 mL glucose media to reach a starting cell density of 104 cells/mL. The cells were then grown in glucose for 12 hr, before being washed to maltose media where they experience a lag phase.
Library Construction Protocol:	10x Genomics, V2 chemistry, For each sequencing run (1 NextSeq & 1 HiSeq with 2 lanes) and each sequencing lane and each sample, three fastq files are provided. The first fastq file corresponds to i7 index (I1, 8bps) and indicates the sample index. The second fastq files corresponds to read 1 (R1, 26bps) and indicates the cell barcode and Unique Molecular Identifier (UMI). The 3rd fastq file corresponds to Read 2 (R2, 98 bps) and corresponds to cDNA sequence.

Molecule Type:	poly(A)+ RNA
Library Source:
Library Layout:	PAIRED
Library Strand:	-
Platform:	ILLUMINA
Instrument Model:	Illumina HiSeq 3000
Strand-Specific:	-

Data Resource	GEN Sample ID	GEN Dataset ID	Project ID	BioProject ID	Sample ID	Sample Name	BioSample ID	Sample Accession	Experiment Accession	Release Date	Submission Date	Update Date	Species	Race	Ethnicity	Age	Age Unit	Gender	Source Name	Tissue	Cell Type	Cell Subtype	Cell Line	Disease	Disease State	Development Stage	Mutation	Phenotype	Case Detail	Control Detail	Growth Protocol	Treatment Protocol	Extract Protocol	Library Construction Protocol	Molecule Type	Library Layout	Strand-Specific	Library Strand	Spike-In	Strategy	Platform	Instrument Model	Cell Number	Reads Number	Gbases	AvgSpotLen1	AvgSpotLen2	Uniq Mapping Rate	Multiple Mapping Rate	Coverage Rate