Summary: In order to better understand the biology of adult schistosomes, we performed single-cell RNAseq on adult male, adult sexually-mature female, and adult virgin female parasites.
Overall Design: Adult parasites were dissociated then live, single cells were sorted by FACS. Single-cell RNAseq libraries were generated from these cells, then these libraries were sequenced. Further data processing was carried out in Seurat as desribed in the associated manuscript.
Strategy: |
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Species: |
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Healthy Condition: |
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Development Stage: |
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Growth Protocol: | - |
Treatment Protocol: | - |
Extract Protocol: | Parasites were dissociated and FACS sorted as described in the associated manuscript |
Library Construction Protocol: | single-cell RNAseq libraries were prepared using a 10x genomics Chromium Controller according to the manufacturer's protocol. RNA-Seq (8bp index, 28bp barcode, 58bp insert) |
Molecule Type: | poly(A)+ RNA |
Library Source: | |
Library Layout: | PAIRED |
Library Strand: | Forward |
Platform: | ILLUMINA |
Instrument Model: | Illumina NextSeq 500 |
Strand-Specific: | Specific |