Gene Expression Nebulas
A data portal of transcriptomic profiles analyzed by a unified pipeline across multiple species

Gene Expression Nebulas

A data portal of transcriptome profiles across multiple species

PRJNA611783: A single-cell RNA-seq atlas of the pathogenic stage of Schistosoma mansoni identifies a key regulator of blood feeding [scRNA-Seq]

Source: NCBI / GSE146736
Submission Date: Mar 10 2020
Release Date: Mar 11 2020
Update Date: Mar 11 2020

Summary: In order to better understand the biology of adult schistosomes, we performed single-cell RNAseq on adult male, adult sexually-mature female, and adult virgin female parasites.

Overall Design: Adult parasites were dissociated then live, single cells were sorted by FACS. Single-cell RNAseq libraries were generated from these cells, then these libraries were sequenced. Further data processing was carried out in Seurat as desribed in the associated manuscript.

GEN Datasets:
GEND000028
Strategy:
Species:
Healthy Condition:
Development Stage:
Protocol
Growth Protocol: -
Treatment Protocol: -
Extract Protocol: Parasites were dissociated and FACS sorted as described in the associated manuscript
Library Construction Protocol: single-cell RNAseq libraries were prepared using a 10x genomics Chromium Controller according to the manufacturer's protocol. RNA-Seq (8bp index, 28bp barcode, 58bp insert)
Sequencing
Molecule Type: poly(A)+ RNA
Library Source:
Library Layout: PAIRED
Library Strand: Forward
Platform: ILLUMINA
Instrument Model: Illumina NextSeq 500
Strand-Specific: Specific
Samples
Basic Information:
Sample Characteristic:
Biological Condition:
Experimental Variables:
Protocol:
Sequencing:
Assessing Quality:
Analysis:
Publications
A single-cell RNA-seq atlas of Schistosoma mansoni identifies a key regulator of blood feeding.
Science (New York, N.Y.) . 2020-09-01 [PMID: 32973030]