Growth Protocol:	-
Treatment Protocol:	Cryopreserved PBMC samples were thawed and resuspended in complete cell culture media at a concentration of 5 × 106 cell/mL and stimulated with 0.5 μg/mL of a SARS-CoV-2 Spike protein peptide pool for 18 h at 37 °C. Spike-reactive CD4+ T cells were identified by expression of the activation markers CD134 and CD69 and isolated by flow cytometric sorting using BD FACSAria II instrument. Cells were sorted directly into 350ul RLT+ buffer supplemented with 1% 2-ME
Extract Protocol:	RNA isolated from RLT+ buffer using a RNeasy Micro spin column according to the manufacuter's protocol
Library Construction Protocol:	cDNA was generated using a SMART-Seq HT Kit (TaKaRa, 634455), and the final Illumina-compatible DNA sequencing libraries prepared using an Illumina Nextera XT DNA Library Preparation kit

Molecule Type:	polyA(+) RNA
Library Source:
Library Layout:	SINGLE
Library Strand:	-
Platform:	ILLUMINA
Instrument Model:	Illumina NextSeq 500
Strand-Specific:	Unspecific

Data Resource	GEN Sample ID	GEN Dataset ID	Project ID	BioProject ID	Sample ID	Sample Name	BioSample ID	Sample Accession	Experiment Accession	Release Date	Submission Date	Update Date	Species	Race	Ethnicity	Age	Age Unit	Gender	Source Name	Tissue	Cell Type	Cell Subtype	Cell Line	Disease	Disease State	Development Stage	Mutation	Phenotype	Case Detail	Control Detail	Growth Protocol	Treatment Protocol	Extract Protocol	Library Construction Protocol	Molecule Type	Library Layout	Strand-Specific	Library Strand	Spike-In	Strategy	Platform	Instrument Model	Cell Number	Reads Number	Gbases	AvgSpotLen1	AvgSpotLen2	Uniq Mapping Rate	Multiple Mapping Rate	Coverage Rate