PRJNA723838: Circular RNA YPEL2 promotes cell proliferation, migration and invasion as a biomaker in cervical cancer

Summary: Circular RNA (circRNA) is newly type of noncoding RNA that are formed by back-splicing of exon and play the important functions in tumorigenesis. However, the research of circRNA is deficient in cervical cancer (CC). We performed high-throughput sequencing on cervical cancer tissues and normal cervical control samples. We discovered the up-regulated circYPEL2 (has_circ_0005400) in cervical cancer and used this as a potential research object through bioinformatics analysis. Finally, through RNA sequencing and bioinformatics technology, we analyzed the downstream targeted genes that may be affected by circYPEL2.

Overall Design: Cervical cancer tissues and normal cervical control samples were collected from 3 cervical cancer patients and 3 normal patients. Total RNA was extracted from these samples, and the RNA was reverse transcribed to cDNA and constructed into a strand-specific library. Illumina HiSeq2000 was performed for sequencing. We designed short interfering RNAs (si-NC, si-circYPEL2-1, si-circYPEL2-2) to transfect into HeLa and SiHa cell lines. After verifying by qRT-PCR, we found the si-circYPEL2-2 is the most efficient. So we used it for three independent replicates to knock down circYPEL2. Total RNA was extracted, and the RNA was reverse transcribed to cDNA and constructed into a strand-specific library. Illumina HiSeq2000 was performed for sequencing.