Gene Expression
Nebulas
Gene Expression NebulasSummary: Assessment of the endothelial transcriptome following exposure to platelet releasated factors from COVID-19 and controls.
Overall Design: Human cardiac microvascular endothelial cells exposed to platelet releasate (7 controls, 7 COVID-19) originating from patients with COVID-19 or controls.
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| Growth Protocol: | - |
| Treatment Protocol: | Human cardiac microvascular endothelial cells exposed to platelet releasate originating from patients with COVID-19 or controls. |
| Extract Protocol: | Direct-zol RNA microspin columns |
| Library Construction Protocol: | Sequencing libraries were barcoded and prepared using the Clontech SMART-Seq HT with Nxt HT kit (Takara Bio USA) as per the manufacturer's instruction |
| Molecule Type: | polyA(+) RNA |
| Library Source: | |
| Library Layout: | PAIRED |
| Library Strand: | - |
| Platform: | ILLUMINA |
| Instrument Model: | Illumina NovaSeq 6000 |
| Strand-Specific: | Unspecific |
| Data Resource | GEN Sample ID | GEN Dataset ID | Project ID | BioProject ID | Sample ID | Sample Name | BioSample ID | Sample Accession | Experiment Accession | Release Date | Submission Date | Update Date | Species | Race | Ethnicity | Age | Age Unit | Gender | Source Name | Tissue | Cell Type | Cell Subtype | Cell Line | Disease | Disease State | Development Stage | Mutation | Phenotype | Case Detail | Control Detail | Growth Protocol | Treatment Protocol | Extract Protocol | Library Construction Protocol | Molecule Type | Library Layout | Strand-Specific | Library Strand | Spike-In | Strategy | Platform | Instrument Model | Cell Number | Reads Number | Gbases | AvgSpotLen1 | AvgSpotLen2 | Uniq Mapping Rate | Multiple Mapping Rate | Coverage Rate |
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