Gene Expression
Nebulas
Gene Expression NebulasSummary: To understand how COVID-19 alters the platelet transcriptome.
Overall Design: A cohort of eight hospitalized COVID-19 patients (n=8) were recruited from NYU Langone Health between May 11-21, 2020. SARS-CoV-2 infection was confirmed by RT-PCR, in accordance with current standards. All COVID-19 patients and , age-, and sex-matched control donors were recruited under study protocols approved by the NYU Langone Health Institutional Review Board. Each study participant or their Legal Authorized Representative gave written informed consent for study enrollment in accordance with the Declaration of Helsinki. For COVID-19 patients, enrollment criteria included age greater than 18, hospital admission, positive SARS-CoV-2 testing, and informed consent. COVID-19 patients were monitored until discharge or death.
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| Growth Protocol: | - |
| Treatment Protocol: | - |
| Extract Protocol: | Direct-zol RNA microspin columns |
| Library Construction Protocol: | Sequencing libraries were barcoded and prepared using the Clontech SMART-Seq HT with Nxt HT kit (Takara Bio USA) as per the manufacturer's instruction |
| Molecule Type: | polyA(+) RNA |
| Library Source: | |
| Library Layout: | PAIRED |
| Library Strand: | - |
| Platform: | ILLUMINA |
| Instrument Model: | Illumina NovaSeq 6000 |
| Strand-Specific: | Unspecific |
| Data Resource | GEN Sample ID | GEN Dataset ID | Project ID | BioProject ID | Sample ID | Sample Name | BioSample ID | Sample Accession | Experiment Accession | Release Date | Submission Date | Update Date | Species | Race | Ethnicity | Age | Age Unit | Gender | Source Name | Tissue | Cell Type | Cell Subtype | Cell Line | Disease | Disease State | Development Stage | Mutation | Phenotype | Case Detail | Control Detail | Growth Protocol | Treatment Protocol | Extract Protocol | Library Construction Protocol | Molecule Type | Library Layout | Strand-Specific | Library Strand | Spike-In | Strategy | Platform | Instrument Model | Cell Number | Reads Number | Gbases | AvgSpotLen1 | AvgSpotLen2 | Uniq Mapping Rate | Multiple Mapping Rate | Coverage Rate |
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