Gene Expression
Nebulas
Gene Expression NebulasSummary: To delineate the in situ immune responses to SARS-CoV-2 viral infection, we performed comprehensive immune profiling in COVID-19 decedents. In this study, we described the immune microenvironment of COVID-19 patients through transcriptome sequencing.
Overall Design: This dataset contains expression from SARS-CoV-2 infected patients and healthy control, various tissues.
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| Growth Protocol: | - |
| Treatment Protocol: | All samples are taken from autopsy specimens, and then bulk RNA is directly extracted for sequencing |
| Extract Protocol: | RNA electrophoresis was extracted from FFPE samples, 200-500bp fragments were recovered, PCR amplified and recovered fragments were constructed to construct a sequencing library. |
| Library Construction Protocol: | The sequencing library construction and sequencing reaction used Illumina's standard procedures (Illumina, USA), and the library size 300bp, the sequencing strategy is PE150. |
| Molecule Type: | rRNA- RNA |
| Library Source: | |
| Library Layout: | PAIRED |
| Library Strand: | Forward |
| Platform: | ILLUMINA |
| Instrument Model: | Illumina NovaSeq 6000 |
| Strand-Specific: | Specific |
| Data Resource | GEN Sample ID | GEN Dataset ID | Project ID | BioProject ID | Sample ID | Sample Name | BioSample ID | Sample Accession | Experiment Accession | Release Date | Submission Date | Update Date | Species | Race | Ethnicity | Age | Age Unit | Gender | Source Name | Tissue | Cell Type | Cell Subtype | Cell Line | Disease | Disease State | Development Stage | Mutation | Phenotype | Case Detail | Control Detail | Growth Protocol | Treatment Protocol | Extract Protocol | Library Construction Protocol | Molecule Type | Library Layout | Strand-Specific | Library Strand | Spike-In | Strategy | Platform | Instrument Model | Cell Number | Reads Number | Gbases | AvgSpotLen1 | AvgSpotLen2 | Uniq Mapping Rate | Multiple Mapping Rate | Coverage Rate |
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