Basic Information

Agrostis stolonifera
Accession: ICG00002
Latin Name: Agrostis stolonifera
Common Name: Creeping bentgrass, Creeping bent, Fiorin, Spreading bent
Taxonomy ID: 63632
Description: Agrostis stolonifera is one of the most widely utilized cool-season grass species for turfgrass and forage in temperate climatic regions and displays moderate cold tolerance but is particularly sensitive to other abiotic stresses such as heat, drought, and salinity. Most studies focused on creeping bentgrass physiological mechanisms involved in heat, salt, and drought stresses, but limited information is available on the underlying molecular mechanisms conferring improved stress tolerance.

Gene Summary

mRNA

ICG00002-1

Studies (1)

ICG00002-1

Abiotic Stresses
Tissues
Temperature Conditions

Internal Control Genes

Gene Symbol Gene Model Application Scenario Accession Number Primers(5' - 3')
(Forward/Reverse)
Size (bp) Annealing Temperature Detection
CACS Clathrin Adaptor Complex
Salinity Stress
Root
Leaf
Drought Stress
Low Temperature Condition
High Temperature Condition
GR280784
F: TAGAGGGTCTGAAGAGGCG
R: CTGGTGTGATCGAAAAGTGG
121 60 SYBR
UPL7 Ubiquitin
Salinity Stress
Root
Leaf
Drought Stress
Low Temperature Condition
High Temperature Condition
DV858635
F: TGTGACTGGATGTTTTCGTGG
R: TTGGTTTTGTATGGGGGGA
162 60 SYBR
PP2A Protein Phosphatase
Salinity Stress
Root
Leaf
Drought Stress
Low Temperature Condition
High Temperature Condition
DV858985
F: AACTTCCTCCAATTCGATCC
R: AACTTCCTCCAATTCGATCC
190 60 SYBR
ACT Actin
Salinity Stress
Root
Leaf
DV857524
F: CCTTAGCACCTTCCAACAGA
R: AAGTGGAAAGACCCAAGACAA
114 60 SYBR
GAPDH Glyceraldehyde-3-Phosphate Dehydrogenase
Salinity Stress
Root
Leaf
High Temperature Condition
GR281777
F: GCCTTCCGTGTTCCTACTGT
R: AAGATGCTGGACCTGCTGTC
191 60 SYBR
PEPKR1 Phosphoenolpyruvate Carboxylase Kinase
Salinity Stress
Root
Leaf
Drought Stress
DV853577
F: ACCGATGTTGTTGGAAGCC
R: ACACCACTCAGCAGAATGTAGAG
116 60 SYBR
EF1a Elongation Factor
Leaf
High Temperature Condition
DV858943
F: CAAGGTTACCAAGGCTGCTG
R: GGTTTTGCCGAACATGAAATAG
170 60 SYBR

Molecular Types:

mRNA

Evaluation Methods

Contact

Name: Zhimin Yang
Email: nauyzm@njau.edu.cn
Institution: College of Agro-grassland Science, Nanjing Agricultural University, Nanjing 210095, China

Reference

Selection of reference genes for quantitative real-time PCR normalization in creeping bentgrass involved in four abiotic stresses.
Chen Y, Hu B, Tan Z, Liu J, Yang Z, Li Z, Huang B.
Plant cell reports. 2015-07-16