ICG00176 Salmo salar - Internal Control Genes Database (ICG)

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Salmo salar

Accession: ICG00176

Latin Name: Salmo salar

Common Name: Atlantic salmon

Taxonomy ID: 8030

Description: Salmo salar is a menber of salmon fish family. This kind of fish is found in the river that flow into the north Atlantic. It is also found in the North Pacific due to human introduction. Atlantic salmon has been significantly reducing because of the recreational and commercial fishing, as well as the habitat destruction. This species is the subject of several national protection efforts.

ICG00176-1

Tissues

Abiotic Stresses

Internal Control Genes

Gene Symbol	Gene Model	Application Scenario	Accession Number	Primers(5' - 3') (Forward/Reverse)	Size (bp)	Annealing Temperature	Detection
EF1AA	Elongation Factor	Gill Liver Head Kidney Spleen Thymus Brain Muscle Posterior Intestine Physiological Stress	AF321836	F: CCCCTCCAGGACGTTTACAAA R: CACACGGCCCACAGGTACA	57	60	TaqMan
EF1AB	Elongation Factor	Gill Liver Head Kidney Spleen Thymus Brain Muscle Posterior Intestine Physiological Stress	BG933853	F: TGCCCCTCCAGGATGTCTAC R: CACGGCCCACAGGTACTG	59	60	TaqMan

Molecular Types:

mRNA

Evaluation Methods

geNorm

Contact

Name: Pål A Olsvik

Email: pal.olsvik@nifes.no

Institution: National Institute of Nutrition and Seafood Research, Nordnesboder,N-5020 Bergen, Norway

Reference

ICG00176-2

Myogenic Cell Culture

Developmental Stage

Internal Control Genes

Gene Symbol	Gene Model	Application Scenario	Accession Number	Primers(5' - 3') (Forward/Reverse)	Size (bp)	Annealing Temperature	Detection
Ef1a	Elongation Factor	Myogenic Satellite Cell Juvenile Individual	BG933853	F: GAATCGGCTATGCCTGGTGAC R: GGATGATGACCTGAGCGGTG	141	60	SYBR
Hprt1	Hypoxanthine Phosphoribosyltransferase	Myogenic Satellite Cell Juvenile Individual	EG866745	F: CCGCCTCAAGAGCTACTGTAAT R: GTCTGGAACCTCAAACCCTATG	255	60	SYBR
Ppia	Peptidylprolyl Isomerase	Myogenic Satellite Cell Juvenile Individual	DY727143	F: CATCCCAGGTTTCATGTGC R: CCGTTCAGCCAGTCAGTGTT	203	60	SYBR
RNApolII	RNA Polymerase	Myogenic Satellite Cell Juvenile Individual	BG936649	F: CCAATACATGACCAAATATGAAAGG R: ATGATGATGGGGATCTTCCTGC	157	60	SYBR

Molecular Types:

mRNA

Evaluation Methods

geNorm

NormFinder

BestKeeper

Contact

Name: Neil I Bower

Email: nib@st-andrews.ac.uk

Institution: Scottish Oceans Institute, School of Biology, University of St Andrews, St Andrews, Fife, KY16 8LB, UK

Reference

ICG00176-3

Tissues

Internal Control Genes

Gene Symbol	Gene Model	Application Scenario	Accession Number	Primers(5' - 3') (Forward/Reverse)	Size (bp)	Annealing Temperature	Detection
ssa-miR-25-3p	MiRNA	Kidney Liver Brain Intestine Heart White Muscle Gill	-	F: CATTGCACTTGTCTCGGTCTGA R: Please contact with the researchers for details.	-	55	SYBR
ssa-miR-455-5p	MiRNA	Kidney Liver Brain Intestine Heart White Muscle Gill	-	F: TATGTGCCCTTGGACTACATCG R: Please contact with the researchers for details.	-	55	SYBR

Molecular Types:

MicroRNA

Evaluation Methods

NormFinder

Contact

Name: Rune Andreassen

Email: Rune.Andreassen@hioa.no

Institution: Faculty of Health Sciences, Oslo and Akershus University College of Applied Sciences, Oslo, Norway

Reference

ICG00176-4

Immune System Model

Immune Relevant Tissues

Biotic Stresses

Viral Infection

Internal Control Genes

Gene Symbol	Gene Model	Application Scenario	Accession Number	Primers(5' - 3') (Forward/Reverse)	Size (bp)	Annealing Temperature	Detection
18S	18S Ribosomal RNA	Gill Liver Spleen Kidney Hindgut Midgut Heart Salmon Anemia Infection	AJ427629	F: TGTGCCGCTAGAGGTGAAATT R: GCAAATGCTTTCGCTTTCG	61	60	SYBR
EF1A	Elongation Factor	Gill Liver Spleen Kidney Hindgut Midgut Heart Salmon Anemia Infection	AF321836	F: CACCACCGGCCATCTGATCTACAA R: TCAGCAGCCTCCTTCTCGAACTTC	77	60	SYBR
RPL1	Ribosomal Protein L10	Gill Liver Spleen Kidney Hindgut Midgut Heart Salmon Anemia Infection	CB516726	F: ACTATGGCTGTCGAGAAGGTGCT R: TGTACTCGAACAGTCGTGGGTCA	120	60	SYBR

Molecular Types:

mRNA

Evaluation Methods

geNorm

Contact

Name: Tor Gjoen

Email: tor.gjoen@farmasi.uio.no

Institution: Department of Pharmaceutical Biosciences, School of Pharmacy, University of Oslo, 0316 Oslo, NorwayDepartment of Pharmaceutical Biosciences, School of Pharmacy, University of Oslo, 0316 Oslo, Norway

Reference

ICG00176-5

Tissues

Abiotic Stresses

Internal Control Genes

Gene Symbol	Gene Model	Application Scenario	Accession Number	Primers(5' - 3') (Forward/Reverse)	Size (bp)	Annealing Temperature	Detection
ssa-miR-23a-3p	MiRNA	Kidney Spleen Liver Muscle Blood Early Response To Handling Early Response To Confinement	MIMAT0032549	F: ATCACATTGCCAGGGATTTCC R: Please contact with the researchers for details.	21	60	SYBR
ssa-miR-99-5p	MiRNA	Kidney Spleen Liver Muscle Blood Early Response To Handling Early Response To Confinement	MIMAT0032718	F: AACCCGTAGATCCGATCTTGTG R: Please contact with the researchers for details.	23	60	SYBR

Molecular Types:

MicroRNA

Evaluation Methods

geNorm

NormFinder

Contact

Name: Rodrigo Vidal

Email: ruben.vidal@usach.cl

Institution: Department of Biology, Universidad de Santiago de Chile. Av. Libertador Bernardo Oiggins 3363, Santiago, Chile.

Basic Information

Gene Summary

MicroRNA

mRNA

Studies (5)

ICG00176-1

Internal Control Genes

Molecular Types:

Evaluation Methods

Contact

Reference

ICG00176-2

Internal Control Genes

Molecular Types:

Evaluation Methods

Contact

Reference

ICG00176-3

Internal Control Genes

Molecular Types:

Evaluation Methods

Contact

Reference

ICG00176-4

Internal Control Genes

Molecular Types:

Evaluation Methods

Contact

Reference

ICG00176-5

Internal Control Genes

Molecular Types:

Evaluation Methods

Contact

Reference

ICG00176-3	ssa-miR-25-3p ssa-miR-455-5p
ICG00176-5	ssa-miR-23a-3p ssa-miR-99-5p

ICG00176-2	Ef1a Hprt1 Ppia RNApolII
ICG00176-4	18S EF1A RPL1
ICG00176-1	EF1AA EF1AB