Basic Information

Gluconacetobacter diazotrophicus
Accession: ICG00205
Latin Name: Gluconacetobacter diazotrophicus
Common Name: -
Taxonomy ID: 33996
Description: Gluconacetobacter diazotrophicus strain PAL5 is a nitrogen-fixing endophytic bacterium originally isolated from sugarcane and later on was found to colonize other plants such as rice, elephant grass, sweet potato, coffee, and pineapple. Currently, G. diazotrophicus has been considered a plant growth-promoting bacterium due to its characteristics of biological nitrogen fixation, phytohormone secretion, solubilization of mineral nutrients and antagonism to phytopathogens.

Gene Summary

mRNA

ICG00205-1

Studies (1)

ICG00205-1

Culture Conditions

Internal Control Genes

Gene Symbol Gene Model Application Scenario Accession Number Primers(5' - 3')
(Forward/Reverse)
Size (bp) Annealing Temperature Detection
rho Rhodopsin
Lgi Medium
NM_003134
F: GCAACTCGGAACTCATCCTC
R: GGACCCACATCTTCGACAGT
132 58 SYBR
23S rRNA 23S Ribosomal RNA
Lgi Medium
NM_003135
F: GTGAGACAGTTCGGTCCCTATC
R: CGATTATCCCGTCCAGACTTAG
150 58 SYBR
rpoD RNA Polymerase
Lgi Medium
NM_003136
F: ACAACGACACCACTCTGCTG
R: CTCCGACGACATCTGATCCT
131 58 SYBR

Molecular Types:

mRNA

Evaluation Methods

Contact

Name: Jean L. Simões-Araújo
Email: jean@cnpab.embrapa.br
Institution: Laboratório de Genética e Bioquímica, Embrapa Agrobiologia, Seropédica, RJ, Brazil

Reference

Identification and validation of reference genes to study the gene expression in Gluconacetobacter diazotrophicus grown in different carbon sources using RT-qPCR.
Galisa PS, da Silva HA, Macedo AV, Reis VM, Vidal MS, Baldani JI, Simões-Araújo JL.
Journal of microbiological methods. 2012-07-16