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Acanthamoeba keratitis

Basic Information

Acanthamoeba keratitis
Accession: ICG10259
Latin Name: Acanthamoeba keratitis
Common Name: NA
Taxonomy ID: 333130
Description: Acanthamoebae are potentially pathogenic organisms, with a highly unique, yet still insufficiently investigated metabolism. Acanthamoebae can easily be cultured in the laboratory and make an attractive model organism for cellular differentiation, evolutionary studies and studies on microbial interactions.

Gene Summary

mRNA

ICG10259-1
18SQV HPRT

Studies (1)

ICG10259-1

Strains
Temperature Conditions
Abiotic Stresses

Internal Control Genes

Gene Symbol Gene Model Application Scenario Accession Number Primers(5' - 3')
(Forward/Reverse)		 Size (bp) Annealing Temperature Detection
18SQV 18S Ribosomal RNA
Strain 1Bu
Strain Spa08
Strain Str16
Strain Neff
Oxidative Stress
High Temperature Condition
Low Temperature Condition
 -
F: CCCAGATCGTTTACCGTGAA
R: TAAATATTAATGCCCCCAACTATCC
 179 59.65 SYBR
HPRT Hypoxanthine Phosphoribosyltransferase
Strain 1Bu
Strain Spa08
Strain Str16
Strain Neff
Oxidative Stress
High Temperature Condition
Low Temperature Condition
 XM_004337011
F: GGAGCGGATCGTTCTCTG
R: ATCTTGGCGTCGACGTGC
 201 58.4 SYBR

Molecular Types:

mRNA

Evaluation Methods

geNorm
NormFinder
BestKeeper

Contact

Name: Julia Walochnik
Email: julia.walochnik@meduniwien.ac.at
Institution: Institute of Specific Prophylaxis und Tropical Medicine, Center for Pathophysiology, Infectiology and Immunology, Medical University of Vienna, Vienna, Australia.

Reference

Validation of reference genes for the normalization of RT-qPCR gene expression in Acanthamoeba spp.
Köhsler M, Leitsch D, Müller N, Walochnik J.
Scientific reports. 2020-06-25
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