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Journal of virology
Jul, 1992;66 (7): 4570-5.

Role of human T-cell leukemia virus type 1 X region proteins in immortalization of primary human lymphocytes in culture.

R Grassmann, S Berchtold, I Radant, M Alt, B Fleckenstein, J G Sodroski, W A Haseltine, U Ramstedt
Author Information
  1. R Grassmann: Institut für Klinische und Molekulare Virologie, Universität Erlangen-Nürnberg, Germany.
PMID: 1351105 DOI: 10.1128/JVI.66.7.4570-4575.1992

Abstract

Human T-cell leukemia virus type 1 (HTLV-1) immortalizes human CD4+ T lymphocytes in culture. Previous studies show that in the context of a herpesvirus saimiri vector, the sequence of the X region at the 3' end of the HTLV-1 genome is also capable of immortalizing CD4+ lymphocytes in the absence of HTLV-1 structural proteins. The X region of HTLV-1 encodes two trans-acting viral proteins, the 42-kDa Tax protein and the 27-kDa Rex protein. Infection of human cord blood cells with herpesvirus saimiri recombinants which contain HTLV-1 X region sequences defective for expression of tax, rex, or both tax and rex demonstrates that tax function is necessary and sufficient for immortalization of primary human CD4+ cord blood lymphocytes in culture in the context of the herpesvirus saimiri vector.

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Grants

  1. CA 36974/NCI NIH HHS

MeSH Term

Base Sequence
CD4-Positive T-Lymphocytes
Cell Transformation, Viral
Cells, Cultured
DNA, Viral
Gene Products, rex
Gene Products, tax
Human T-lymphotropic virus 1
Humans
Molecular Sequence Data
Mutagenesis
Transcription, Genetic

Chemicals

DNA, Viral
Gene Products, rex
Gene Products, tax
Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Word Cloud

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