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Histochemistry
1990;93 (5): 459-68.

Two-colour immunofluorescence marker study of pleomorphic adenomas.

P S Thrane, D R Roop, L M Sollid, H S Huitfeldt, P Brandtzaeg
Author Information
  1. P S Thrane: Laboratory for Immunohistochemistry and Immunopathology (LIIPAT), Medical Faculty, National Hospital, Rikshospitalet, Oslo, Norway.
PMID: 2158957 DOI: 10.1007/BF00266401

Abstract

Extensive use of two-colour immunofluorescence staining for various cell markers in pleomorphic adenoma, revealed three consistent phenotypic features: (1) keratin polypeptide No. 14, which was virtually restricted to myoepithelial cells (MEC) in normal salivary glands, appeared in a large fraction of the tumour cells, suggesting that the principal neoplastic element is derived from MEC or their immediate precursors; (2) a complex co-expression pattern of various cell markers was found, with extensive concurrence of keratin and vimentin in strands of MEC-like and myxoid tumour cells, probably reflecting different degrees of tumour cell differentiation; and (3) two phenotypically distinctive dendritic cell populations were identified, one consisting of keratin positive tumour cells and the other of HLA-DR positive but keratin negative stromal cells. The significance of these findings with regard to the histogenesis and complex morphology of pleomorphic adenoma is discussed.

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MeSH Term

Adenoma, Pleomorphic
Adult
Aged
Biomarkers, Tumor
Female
Fluorescent Antibody Technique
HLA-DR Antigens
Humans
Intermediate Filament Proteins
Male
Middle Aged
Parotid Neoplasms
S100 Proteins
von Willebrand Factor

Chemicals

Biomarkers, Tumor
HLA-DR Antigens
Intermediate Filament Proteins
S100 Proteins
von Willebrand Factor
Journal Article Research Support, Non-U.S. Gov't

Word Cloud

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