Crystallization and preliminary X-ray analysis of porin from Rhodobacter capsulatus.

Advanced Search

U Nestel, T Wacker, D Woitzik, J Weckesser, W Kreutz, W Welte

Author Information

U Nestel: Institut für Biophysik und Strahlenbiologie der Albert-Ludwigs-Universität, Freiburg i.Br., FRG.

PMID: 2536626 DOI: 10.1016/0014-5793(89)80511-3

Porin monomers of the phototrophic bacterium Rhodobacter capsulatus were purified. Crystals were obtained from a solution of porin solubilized with the detergent octyltetraoxyethylene within 5 days using the vapor phase equilibration technique. The crystals were rhombohedral with an edge length of 0.4 mm. The space group was trigonal R3 with unit cell constants of a = b = 95 A, c = 147 A. Reflexions were observed to 3.2 A.

Bacterial Outer Membrane Proteins

Crystallography

Gram-Negative Bacteria

Molecular Weight

Porins

Protein Conformation

X-Ray Diffraction

Bacterial Outer Membrane Proteins

Porins

Journal Article Research Support, Non-U.S. Gov't

OpenLB
Open Library of Bioscience