Virus inactivation in hemoglobin solutions by heat.

T N Estep, M K Bechtel, T J Miller, A Bagdasarian
Author Information
  1. T N Estep: Travenol Laboratories, Inc., Round Lake, Illinois 60073.

Abstract

To determine the feasibility of heat treating hemoglobin solutions to inactivate viruses, we performed experiments on the thermal stability of this protein and found that the structure and function of deoxyhemoglobin are well preserved during incubation at 60 degrees C for 10 hours at a pH of 7.5. Model viruses and the human immunodeficiency virus were rapidly inactivated under these conditions. The results imply that incubation at 60 degrees C is a practical method for inactivating viruses in hemoglobin solutions.

MeSH Term

Dithionite
Drug Contamination
HIV
Hemoglobins
Herpesvirus 1, Suid
Hot Temperature
Humans
Oxygen
Poliovirus
Sindbis Virus
Solutions
Spectrophotometry
Virus Physiological Phenomena

Chemicals

Hemoglobins
Solutions
hemoglobin, stroma free
Dithionite
Oxygen

Word Cloud

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