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Molecular & general genetics : MGG
May, 1987;207 (2-3): 430-4.

Effect of DNA superhelicity on transcription termination.

E R Rosenthal, J M Calvo
Author Information
PMID: 3302600 DOI: 10.1007/BF00331611

Abstract

Restriction fragments containing either leut (a rho-independent transcription termination site) and/or leut' (a rho-dependent transcription termination site) were cloned into plasmid pOL4. Treatment of plasmid-containing Escherichia coli strains with coumermycin resulted in loss of in vivo plasmid superhelicity 10 min after antibiotic addition. Galactokinase levels specified by these plasmid-containing strains were the same regardless of whether functional DNA gyrase was present. These results suggest that transcription termination is unaffected by the superhelical state of DNA.

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Grants

  1. 14340/PHS HHS

MeSH Term

Acetyltransferases
Aminocoumarins
Anti-Bacterial Agents
Base Sequence
Chloramphenicol O-Acetyltransferase
Coumarins
DNA, Superhelical
Escherichia coli
Galactokinase
Plasmids
Transcription, Genetic

Chemicals

Aminocoumarins
Anti-Bacterial Agents
Coumarins
DNA, Superhelical
Acetyltransferases
Chloramphenicol O-Acetyltransferase
Galactokinase
coumermycin
Journal Article Research Support, U.S. Gov't, P.H.S.

Word Cloud

Created with Highcharts 10.0.0transcriptionterminationDNAsiteplasmidplasmid-containingstrainssuperhelicityRestrictionfragmentscontainingeitherleutrho-independentand/orleut'rho-dependentclonedpOL4TreatmentEscherichiacolicoumermycinresultedlossvivo10minantibioticadditionGalactokinaselevelsspecifiedregardlesswhetherfunctionalgyrasepresentresultssuggestunaffectedsuperhelicalstateEffect

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