Mass spectrometric analysis of chitin oligosaccharides produced by Rhizobium NodC protein in Escherichia coli.
E Kamst, K M van der Drift, J E Thomas-Oates, B J Lugtenberg, H P Spaink
Author Information
E Kamst: Clusius Laboratory, Institute of Molecular Plant Sciences, Leiden University, The Netherlands.
A system for studying the in vivo activity of Rhizobium NodC protein in Escherichia coli has been developed. Using thin-layer chromatography, high-performance liquid chromatography, and mass spectrometry, we show that in this system R. leguminosarum bv. viciae NodC protein directs the synthesis of chitinpentaose, chitintetraose, chitintriose, and two as yet unidentified modified chitin oligosaccharides.
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Bacterial Proteins
Carbohydrate Sequence
Chitin
Chromatography, High Pressure Liquid
Chromatography, Thin Layer
Escherichia coli
Mass Spectrometry
Molecular Sequence Data
N-Acetylglucosaminyltransferases
Oligosaccharides
Recombinant Proteins
Rhizobium leguminosarum
Trisaccharides
Bacterial Proteins
Oligosaccharides
Recombinant Proteins
Trisaccharides
Chitin
tetra-N-acetylchitotetraose
N,N',N'',N''',N''''-pentaacetylchitinpentaose
N,N',N''-triacetylchitotriose
N-Acetylglucosaminyltransferases
NodC protein, Rhizobiales
