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Plant molecular biology
Mar, 1993;21 (6): 1109-19.

Transposition pattern of a modified Ds element in tomato.

C M Rommens, T R Munyikwa, B Overduin, H J Nijkamp, J Hille
Author Information
  1. C M Rommens: Department of Genetics, Free University, Amsterdam, Netherlands.
PMID: 8098229 DOI: 10.1007/BF00023607

Abstract

Several aspects of transposition of an in vitro modified Ds element are described. This Ds element, designated Ds-r, is equipped with bacterial plasmid sequences and can, therefore, be rescued from the plant genome. Our results indicate that the Ds-r element has a 'late' timing of transposition from T-DNAs. This feature of the element might be advantageous for tagging experiments because it leads to independently transposed germinally transmitted elements. Furthermore, it is shown that Ds-r transposition generates clusters of insertions, indicating that 'genes to be tagged' should be located in genomic regions covered by insertions.

References

  1. Nature. 1984 Jan 12-18;307(5947):127-30 [PMID: 6318121]
  2. Cell. 1991 Aug 9;66(3):465-71 [PMID: 1651170]
  3. Nucleic Acids Res. 1990 Jan 11;18(1):182 [PMID: 2308825]
  4. Genetics. 1992 May;131(1):199-209 [PMID: 1317315]
  5. Mol Gen Genet. 1992 Feb;231(3):433-41 [PMID: 1311405]
  6. EMBO J. 1987 Jun;6(6):1555-63 [PMID: 16453772]
  7. Genetics. 1984 Oct;108(2):471-85 [PMID: 17246235]
  8. Cell. 1983 Nov;35(1):235-42 [PMID: 6313225]
  9. Genetics. 1987 Sep;117(1):109-16 [PMID: 2822530]
  10. Genetics. 1973 Feb;73(2):273-96 [PMID: 17248592]
  11. EMBO J. 1989 Jan;8(1):5-13 [PMID: 16453869]
  12. Genomics. 1987 Oct;1(2):174-81 [PMID: 3692487]
  13. Proc Natl Acad Sci U S A. 1986 Jul;83(13):4844-8 [PMID: 16593722]
  14. Plant Cell. 1990 Aug;2(8):709-21 [PMID: 1967055]
  15. Plant Cell. 1990 Aug;2(8):723-30 [PMID: 1967056]
  16. Cell. 1984 Dec;39(2 Pt 1):253-9 [PMID: 6094008]
  17. Plant Mol Biol. 1992 Oct;20(2):177-98 [PMID: 1327269]
  18. Plant Mol Biol. 1991 Mar;16(3):449-61 [PMID: 1654159]
  19. Cold Spring Harb Symp Quant Biol. 1951;16:13-47 [PMID: 14942727]
  20. Plant Mol Biol. 1992 Oct;20(1):61-70 [PMID: 1355371]
  21. EMBO J. 1985 Sep;4(9):2145-51 [PMID: 15938047]
  22. Plant J. 1992 Mar;2(2):173-9 [PMID: 1338773]
  23. Genetics. 1986 Apr;112(4):887-98 [PMID: 17246322]
  24. J Mol Biol. 1990 Oct 5;215(3):403-10 [PMID: 2231712]
  25. Plant Mol Biol. 1991 May;16(5):753-70 [PMID: 1859863]
  26. Cell. 1990 Aug 10;62(3):515-25 [PMID: 2165865]
  27. Cell. 1984 Jun;37(2):635-43 [PMID: 6327080]
  28. Genetics. 1989 Jun;122(2):447-57 [PMID: 17246501]
  29. Cell. 1983 Aug;34(1):25-35 [PMID: 6309410]
  30. Proc Natl Acad Sci U S A. 1990 May;87(9):3513-7 [PMID: 2185473]
  31. Mol Gen Genet. 1991 Nov;230(1-2):329-31 [PMID: 1660568]
  32. Plant Cell. 1991 May;3(5):473-482 [PMID: 12324601]
  33. Genetics. 1991 Nov;129(3):833-44 [PMID: 1684332]
  34. Genetics. 1992 Mar;130(3):665-76 [PMID: 1312981]
  35. Science. 1989 Apr 14;244(4901):204-7 [PMID: 17835353]
  36. EMBO J. 1988 Aug;7(8):2295-9 [PMID: 16453856]
  37. Genetics. 1992 Aug;131(4):939-56 [PMID: 1325389]
  38. Science. 1986 Oct 10;234(4773):210-1 [PMID: 17746481]

MeSH Term

Base Sequence
Blotting, Southern
DNA
DNA Transposable Elements
DNA, Bacterial
Molecular Sequence Data
Plants
Polymorphism, Restriction Fragment Length

Chemicals

DNA Transposable Elements
DNA, Bacterial
T-DNA
DNA
Journal Article

Word Cloud

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