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Proceedings of the National Academy of Sciences of the United States of America
Oct 15, 1993;90 (20): 9315-9.

Multiple nucleosome positioning with unique rotational setting for the Saccharomyces cerevisiae 5S rRNA gene in vitro and in vivo.

M Buttinelli, E Di Mauro, R Negri
Author Information
  1. M Buttinelli: Centro Acidi Nucleici, Consiglio Nazionale delle Ricerche, Rome, Italy.
PMID: 8415699 DOI: 10.1073/pnas.90.20.9315

Abstract

A simple no-background assay was developed for high-resolution in vivo analysis of yeast chromatin. When applied to Saccharomyces cerevisiae 5S rRNA genes (5S rDNA), this analysis shows that nucleosomes completely cover this chromosomal region, occupying alternative positions characterized by a unique helical phase. This supports the notion that sequence-intrinsic rotational signals are the major determinant of nucleosome localization. Nucleosomal core particles reconstituted in vitro occupy the same positions and have the same helically phased distribution observed in vivo, as determined by mapping of exonuclease III-resistant borders, mapping by restriction cleavages, and by DNase I and hydroxyl-radical digestion patterns.

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MeSH Term

Binding Sites
Chromatin
DNA, Ribosomal
Exodeoxyribonucleases
Histones
Micrococcal Nuclease
Nucleosomes
RNA, Ribosomal, 5S
Restriction Mapping
Saccharomyces cerevisiae

Chemicals

Chromatin
DNA, Ribosomal
Histones
Nucleosomes
RNA, Ribosomal, 5S
Exodeoxyribonucleases
exodeoxyribonuclease III
Micrococcal Nuclease
Journal Article Research Support, Non-U.S. Gov't

Word Cloud

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