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Journal of bacteriology
Oct, 1996;178 (19): 5803-5.

Evidence that TolC is required for functioning of the Mar/AcrAB efflux pump of Escherichia coli.

J A Fralick
Author Information
  1. J A Fralick: Department of Microbiology and Immunology, Texas Tech University Health Sciences Center, Lubbock 79403, USA. micjaf@ttuhsc.edu
PMID: 8824631 DOI: 10.1128/jb.178.19.5803-5805.1996

Abstract

A study examining the influence of TolC on AcrA, AcrR, and MarR1 mutants indicates that functional TolC is required for the operation of the AcrAB efflux system and for the expression of the Mar phenotype. That the effect of TolC on the AcrAB pump is not regulatory in nature is shown by studies measuring the influence of a tolC::Tn10 insertion mutation on the expression of an acrA::lacZ reporter fusion. These results are compatible with the hypothesis that TolC is a component of the AcrAB efflux complex.

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MeSH Term

Acriflavine
Bacterial Outer Membrane Proteins
Bacterial Proteins
Biological Transport
Carrier Proteins
Drug Resistance, Microbial
Drug Resistance, Multiple
Escherichia coli
Escherichia coli Proteins
Lipoproteins
Membrane Proteins
Membrane Transport Proteins
Multidrug Resistance-Associated Proteins
Novobiocin
Repressor Proteins
Sodium Dodecyl Sulfate

Chemicals

AcrA protein, E coli
AcrB protein, E coli
Bacterial Outer Membrane Proteins
Bacterial Proteins
Carrier Proteins
Escherichia coli Proteins
Lipoproteins
MarR protein, E coli
Membrane Proteins
Membrane Transport Proteins
Multidrug Resistance-Associated Proteins
Repressor Proteins
tolC protein, E coli
Novobiocin
Acriflavine
Sodium Dodecyl Sulfate
Journal Article Research Support, Non-U.S. Gov't
Article has an altmetric score of 3

Word Cloud

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